中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2014年
8期
1487-1491
,共5页
钟海%白茫茫%李健%徐东宝
鐘海%白茫茫%李健%徐東寶
종해%백망망%리건%서동보
动静脉瘘%模型,动物%血管内皮生长因子类%转化生长因子β
動靜脈瘺%模型,動物%血管內皮生長因子類%轉化生長因子β
동정맥루%모형,동물%혈관내피생장인자류%전화생장인자β
Arteriovenous fistula%Models,animal%Vascular endothelial growth factors%Transforming growth factor beta
目的：建立兔颈动静脉瘘模型，观察其影像学和病理学变化，探讨其作为介入治疗模型的可行性。方法30只新西兰大白兔随机分为三组：（1）对照组10只，解剖分离但不吻合兔左侧颈总动脉和颈外静脉；（2）实验Ⅰ组（2周）与实验Ⅱ组（4周）各10只，用显微技术吻合兔左侧颈总动脉和颈外静脉；对照组于术后4周，两实验组分别于术后2周及4周行数字减影血管造影（DSA）观察后，取颈外静脉做血管内皮生长因子（VEGF）及转化生长因子-β（TGF-β）的免疫组化染色及HE染色。结果与对照组相比，实验组造影显示颈外静脉扩张、增粗，产生盗血现象；与对照组相比，免疫组化染色显示实验组TGF-β阳性表达显著增多（P＜0.01），VEGF阳性表达轻度增多（P＞0.05）；与对照组相比，HE染色可见实验组颈外静脉内膜显著增厚（P＜0.01）。结论显微吻合兔颈总动脉-颈外静脉建立动静脉瘘模型，其影像学和病理学变化符合模拟硬脑膜动静脉瘘介入治疗模型的要求，可用于介入实验研究。
目的：建立兔頸動靜脈瘺模型，觀察其影像學和病理學變化，探討其作為介入治療模型的可行性。方法30隻新西蘭大白兔隨機分為三組：（1）對照組10隻，解剖分離但不吻閤兔左側頸總動脈和頸外靜脈；（2）實驗Ⅰ組（2週）與實驗Ⅱ組（4週）各10隻，用顯微技術吻閤兔左側頸總動脈和頸外靜脈；對照組于術後4週，兩實驗組分彆于術後2週及4週行數字減影血管造影（DSA）觀察後，取頸外靜脈做血管內皮生長因子（VEGF）及轉化生長因子-β（TGF-β）的免疫組化染色及HE染色。結果與對照組相比，實驗組造影顯示頸外靜脈擴張、增粗，產生盜血現象；與對照組相比，免疫組化染色顯示實驗組TGF-β暘性錶達顯著增多（P＜0.01），VEGF暘性錶達輕度增多（P＞0.05）；與對照組相比，HE染色可見實驗組頸外靜脈內膜顯著增厚（P＜0.01）。結論顯微吻閤兔頸總動脈-頸外靜脈建立動靜脈瘺模型，其影像學和病理學變化符閤模擬硬腦膜動靜脈瘺介入治療模型的要求，可用于介入實驗研究。
목적：건립토경동정맥루모형，관찰기영상학화병이학변화，탐토기작위개입치료모형적가행성。방법30지신서란대백토수궤분위삼조：（1）대조조10지，해부분리단불문합토좌측경총동맥화경외정맥；（2）실험Ⅰ조（2주）여실험Ⅱ조（4주）각10지，용현미기술문합토좌측경총동맥화경외정맥；대조조우술후4주，량실험조분별우술후2주급4주행수자감영혈관조영（DSA）관찰후，취경외정맥주혈관내피생장인자（VEGF）급전화생장인자-β（TGF-β）적면역조화염색급HE염색。결과여대조조상비，실험조조영현시경외정맥확장、증조，산생도혈현상；여대조조상비，면역조화염색현시실험조TGF-β양성표체현저증다（P＜0.01），VEGF양성표체경도증다（P＞0.05）；여대조조상비，HE염색가견실험조경외정맥내막현저증후（P＜0.01）。결론현미문합토경총동맥-경외정맥건립동정맥루모형，기영상학화병이학변화부합모의경뇌막동정맥루개입치료모형적요구，가용우개입실험연구。
Objective To construct a rabbit model of neck arteriovenous fistula, observe the changes in imaging and pathology, and explore the feasibility as intervention model. Methods 30 New Zealand rabbits were randomly divided into 3 groups:(1) 10 rabbits in control group, but not anastomosis with dissection of the left common carotid artery(CCA) and external jugular vein(EJV);(2) the experimentⅠgroup(two weeks) and the experiment Ⅱ group(four weeks) with 10 rabbits in each group, was used microsurgical technique to anastomosis with the CCA and EJV; Digital subtraction angiography (DSA) observation after operation was done in the control group at 4 weeks and the experimentⅠgroup at 2 weeks and the experiment Ⅱ group at 4 weeks, the external jugular vein was taken, and immunohistochemical staining about vascular endothelial growth factor(VEGF) and transforming growth factor-β (TGF-β) and HE staining was done. Results Compared with control group, the experimental group showed EJV was dilation and thickening, and had a"steal blood"phenomenon in the image;compared with control group, immunohistochemical staining of the experiment group showed positive expression of TGF-β increased significantly(P<0.01), the positive expression of VEGF slightly increased(P>0.05);compared with control group, HE staining of the experiment group showed EJV intimal was thickening(P<0.01). Conclusions A arteriovenous fistula model was constructed by using microsurgical technique to anastomosis with the left CCA and EJV, its changes of imaging and pathological to meet the requirements of simulation of dural arteriovenous fistula treatment model, can be used for interventional experimental study.
