中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2014年
8期
1468-1471
,共4页
高志林%张芝娟%马粱明
高誌林%張芝娟%馬粱明
고지림%장지연%마량명
多发性骨髓瘤%细胞周期%P16蛋白%STI571%亚砷酸
多髮性骨髓瘤%細胞週期%P16蛋白%STI571%亞砷痠
다발성골수류%세포주기%P16단백%STI571%아신산
Multiple myeloma%Cell cycle%P16 protein%STI571%As2O3
目的：研究亚砷酸（As2O3）、STI571联用对多发性骨髓瘤（MM）细胞周期及其调节蛋白表达的影响，为As2O3和STI571联合应用于临床提供理论依据。方法用MTT法检测细胞生长抑制率；Western blot方法检测二者对细胞周期负性调控因子P16蛋白的表达情况；用流式细胞仪对干预72 h的SP2/0细胞进一步进行细胞周期分析，以明确该蛋白所影响的细胞周期调控点。结果在As2O3和（或）STI571作用下SP2/0细胞生长受抑伴随活力下降，Western blot方法检测出P16蛋白表达呈时间剂量依赖关系，流式细胞仪DNA含量分析发现As2O3、STI571使SP2/0细胞主要阻滞于G1期，未出现凋亡峰。结论 As2O3和STI571均有抑制SP2/0细胞增殖和上调细胞周期抑制蛋白P16的表达，两药联用效果更佳。
目的：研究亞砷痠（As2O3）、STI571聯用對多髮性骨髓瘤（MM）細胞週期及其調節蛋白錶達的影響，為As2O3和STI571聯閤應用于臨床提供理論依據。方法用MTT法檢測細胞生長抑製率；Western blot方法檢測二者對細胞週期負性調控因子P16蛋白的錶達情況；用流式細胞儀對榦預72 h的SP2/0細胞進一步進行細胞週期分析，以明確該蛋白所影響的細胞週期調控點。結果在As2O3和（或）STI571作用下SP2/0細胞生長受抑伴隨活力下降，Western blot方法檢測齣P16蛋白錶達呈時間劑量依賴關繫，流式細胞儀DNA含量分析髮現As2O3、STI571使SP2/0細胞主要阻滯于G1期，未齣現凋亡峰。結論 As2O3和STI571均有抑製SP2/0細胞增殖和上調細胞週期抑製蛋白P16的錶達，兩藥聯用效果更佳。
목적：연구아신산（As2O3）、STI571련용대다발성골수류（MM）세포주기급기조절단백표체적영향，위As2O3화STI571연합응용우림상제공이론의거。방법용MTT법검측세포생장억제솔；Western blot방법검측이자대세포주기부성조공인자P16단백적표체정황；용류식세포의대간예72 h적SP2/0세포진일보진행세포주기분석，이명학해단백소영향적세포주기조공점。결과재As2O3화（혹）STI571작용하SP2/0세포생장수억반수활력하강，Western blot방법검측출P16단백표체정시간제량의뢰관계，류식세포의DNA함량분석발현As2O3、STI571사SP2/0세포주요조체우G1기，미출현조망봉。결론 As2O3화STI571균유억제SP2/0세포증식화상조세포주기억제단백P16적표체，량약련용효과경가。
Objective To explore the effects of STI571 in combination with As2O3 on proliferation and expression of cyclin dependent kinase inhibitors (CDKIs) of the SP2/0 cells and provide theoretical basis for clinical application. Methods Cell proliferation was detected by MTT methods;Western blot technique was used to detect the expression of cyclin dependent kinase inhibitors (P16 protein) in SP2/0 cell;the DNA content of MM cell line SP2/0 was analysed by flow cytometry after exposure to As2O3 and/or STI571. Results The proliferation of SP2/0 cells was inhibited and the viability of SP2/0 was decreased after exposure to As2O3 and/or STI571. Western blot result showed the expression of P16 protein has significant difference in the different density and different time of As2O3 and/or STI571 groups in the SP2/0 cell. DNA flow cytometry analysis showed that As2O3 and/or STI571 induced most of SP2/0 cells arrest at G1 phase and decrease significantly in S phase. Conclusion One of the pharmacological mechanisms of STI571 combination with As2O3 is to activate or upregulate the expression of protein P16 and consequently affect cell proliferation cycle.
