CAJ | 학술논문

建立了虾籽中柠檬黄、日落黄等10种合成色素及其色淀的高效液相色谱测定方法。样品以氢氧化钠-甲醇溶液提取，加入EDTA络合色淀中的铝离子。采用symmetry C18色谱柱，以甲醇-20 mmol/L醋酸铵为流动相梯度洗脱，二极管阵列检测器测定，外标法定量。10种色素在0．5～20．0 mg/kg范围内线性良好，相关系数均大于0．9999，加标回收率82．4％～107．9％，相对标准偏差0．445％～6．310％，检出限为0．5～1．0 mg/kg。
건립료하자중저몽황、일락황등10충합성색소급기색정적고효액상색보측정방법。양품이경양화납-갑순용액제취，가입EDTA락합색정중적려리자。채용symmetry C18색보주，이갑순-20 mmol/L작산안위류동상제도세탈，이겁관진렬검측기측정，외표법정량。10충색소재0．5～20．0 mg/kg범위내선성량호，상관계수균대우0．9999，가표회수솔82．4％～107．9％，상대표준편차0．445％～6．310％，검출한위0．5～1．0 mg/kg。
A simultaneous determination method of 10 kinds of synthetic colorants , such as tatrazine and sunset yellow , and their aluminum lakes in shrimp eggs was established by high performance liquid chromatog -raphy.The samples were extracted by methanol -NaOH solution, and the aluminium ions were removed by complexation with EDTA.The extracts were separated with symmetry C18(25 cm ×4.6 cm ×5μm)column u-sing methanol-20 mmol/L ammonium acetate as mobile phase .The target compounds were confirmed by di-ode-array detection and quantified by the external standard method .The linear relationships of 10 synthetic colorants were good in the range of 0.5~20.0 mg/kg with correlation coefficients more than 0.9999.The re-covery of standard addition ranged form 82.4%to 107.9%with the relative standard deviation (RSD) in the range of 0.445%~6.310%.And the detection limit was in the range of 0.5~1.0 mg/kg.