山东农业科学
山東農業科學
산동농업과학
SHANGDONG AGRICULTURAL SCIENCES
2014年
1期
86-88
,共3页
刘永亮%苗江涛%孙欢欢%尹成林%丛国强%高克祥
劉永亮%苗江濤%孫歡歡%尹成林%叢國彊%高剋祥
류영량%묘강도%손환환%윤성림%총국강%고극상
南瓜苗%腐烂病%病原鉴定%芽枝霉属%药剂防治
南瓜苗%腐爛病%病原鑒定%芽枝黴屬%藥劑防治
남과묘%부란병%병원감정%아지매속%약제방치
Pumpkin seedling%Rot disease%Pathogen identification%Cladosporium%Pesticide control
通过组织分离法,从温室大棚内发生南瓜苗期腐烂病的植株中分离得到一株菌株。采用南瓜茎人工接种试验进行致病性测定,确认该菌株为病原菌。经形态学鉴定,初步将该病原菌鉴定为瓜枝孢霉( Cladosporium cucumerinum),编号为Cc-1。孢子萌发试验及温室防病试验结果表明:62.25%腈菌唑·福美双可湿性粉剂800倍液与75%百菌清可湿性粉剂600倍液对真菌Cc-1的孢子萌发抑制率均高于90%,温室防治效果分别为76.11%与75.50%。
通過組織分離法,從溫室大棚內髮生南瓜苗期腐爛病的植株中分離得到一株菌株。採用南瓜莖人工接種試驗進行緻病性測定,確認該菌株為病原菌。經形態學鑒定,初步將該病原菌鑒定為瓜枝孢黴( Cladosporium cucumerinum),編號為Cc-1。孢子萌髮試驗及溫室防病試驗結果錶明:62.25%腈菌唑·福美雙可濕性粉劑800倍液與75%百菌清可濕性粉劑600倍液對真菌Cc-1的孢子萌髮抑製率均高于90%,溫室防治效果分彆為76.11%與75.50%。
통과조직분리법,종온실대붕내발생남과묘기부란병적식주중분리득도일주균주。채용남과경인공접충시험진행치병성측정,학인해균주위병원균。경형태학감정,초보장해병원균감정위과지포매( Cladosporium cucumerinum),편호위Cc-1。포자맹발시험급온실방병시험결과표명:62.25%정균서·복미쌍가습성분제800배액여75%백균청가습성분제600배액대진균Cc-1적포자맹발억제솔균고우90%,온실방치효과분별위76.11%여75.50%。
A strain was isolated from pumpkin seedlings with rot disease in greenhouse by tissue isola -tion.The strain was determined as pathogen by artificial inoculation and pathogenicity test on pumpkin stem . The pathogen was preliminarily identified as Cladosporium cucumerinum by morphological identification , named Cc-1.The results of spore germination and control test in greenhouse showed that the inhibition rate of 800 times diluent liquid of 62 .25%myclobutanil and thirm WP and 600 times diluent liquid of 75%chlo-rothalonil WP on fungus Cc -1 spore germination were both over 90%, and the control effect in greenhouse was 76 .11%and 75 .50%respectively .