CAJ | 학술논문

细胞质雄性不育（CMS）在作物遗传育种研究中占有重要地位，多种植物的细胞质雄性不育均与线粒体基因结构变异及新嵌合基因的产生有关。为探索线粒体atp6基因与洋葱CMS的关系，以13份不育系和11份保持系为材料克隆洋葱atp6基因编码序列，测序结果表明不同材料atp6基因间存在一个C/A多态性位点，该多态性位点在不育系和保持系间随机出现，而不是不育系与保持系的特征差异。蛋白质分析表明这一多态性位点的变异并未改变atp6蛋白跨膜结构。进一步的蛋白比对分析揭示了atp6蛋白在物种间有一定的保守性，特别是第IV跨膜结构域的Arg在所有物种中是高度保守的，也是H＋转运所必需的。基于atp6蛋白序列的系统进化树显示细菌和病毒位于树的基部，其次是真菌、低等藻类以及原生生物，然后高等藻类、苔藓和蕨类，高等绿色开花植物处于树的顶端，单子叶植物处于树的最顶端。
세포질웅성불육（CMS）재작물유전육충연구중점유중요지위，다충식물적세포질웅성불육균여선립체기인결구변이급신감합기인적산생유관。위탐색선립체atp6기인여양총CMS적관계，이13빈불육계화11빈보지계위재료극륭양총atp6기인편마서렬，측서결과표명불동재료atp6기인간존재일개C/A다태성위점，해다태성위점재불육계화보지계간수궤출현，이불시불육계여보지계적특정차이。단백질분석표명저일다태성위점적변이병미개변atp6단백과막결구。진일보적단백비대분석게시료atp6단백재물충간유일정적보수성，특별시제IV과막결구역적Arg재소유물충중시고도보수적，야시H＋전운소필수적。기우atp6단백서렬적계통진화수현시세균화병독위우수적기부，기차시진균、저등조류이급원생생물，연후고등조류、태선화궐류，고등록색개화식물처우수적정단，단자협식물처우수적최정단。
Cytoplasm male-sterility ( CMS ) is very important in crop genetic breeding .Usually , this phenomenon is associated with structural variation of mitochondrial gene or formation of novel chimeric gene . In order to explore the relationship between atp6 gene and onion CMS , the coding sequence of atp6 gene was cloned from 13 onion sterile lines and 11 maintainer lines .Sequencing results showed that there was one poly-morphic locus ( C/A) in different onion lines .This polymorphic locus randomly appeared in sterile and main-tainer lines , which was not the characteristic difference between two lines .Protein analysis indicated that the variation of the polymorphic locus didn ’ t change the transmembrane structure of atp 6 protein.Further phylo-genetic analysis revealed that atp 6 protein was conserved among different species .Especially the Arg in the fourth transmembrane domain , which was necessary during the H +transport process , was highly conserved . The phylogenetic tree based on atp 6 protein sequence showed that bacteria and viruses were at the base of the tree, followed by fungi , algae and protest , then higher algae , mosses and ferns , higher green flowering plants, especially monocots, were at the top of the tree.