CAJ | 학술논문

SOS2（Salt Overly Sensitive 2）是植物中一类耐盐相关基因的统称，在植物对盐害的响应及适应过程中具有重要作用。本试验以花生AhSOS2基因全长cDNA为模板，经PCR扩增获得AhSOS2的蛋白编码区，将该区段与表达载体pET-28a（＋）融合，构建获得原核表达载体pET-28a-AhSOS2，并进一步在大肠杆菌BL21（DE3）中进行表达。经1．0 mmol/L IPTG诱导后，获得一条约51 kD的融合蛋白条带，且随着IPTG诱导时间的延长，蛋白表达量逐渐提高，诱导8h可获得较高的蛋白表达水平。
SOS2（Salt Overly Sensitive 2）시식물중일류내염상관기인적통칭，재식물대염해적향응급괄응과정중구유중요작용。본시험이화생AhSOS2기인전장cDNA위모판，경PCR확증획득AhSOS2적단백편마구，장해구단여표체재체pET-28a（＋）융합，구건획득원핵표체재체pET-28a-AhSOS2，병진일보재대장간균BL21（DE3）중진행표체。경1．0 mmol/L IPTG유도후，획득일조약51 kD적융합단백조대，차수착IPTG유도시간적연장，단백표체량축점제고，유도8h가획득교고적단백표체수평。
SOS2 ( Salt Overly Sensitive 2 ) is a kind of salt-tolerant related genes in plant .It plays an im-portant role in the response and adaptation process of plants to salt damage .Using the full-length cDNA of AhSOS2 gene as template , its coding region was amplified by PCR method .The recombined prokaryotic ex-pression vector pET-28a-AhSOS2 was constructed successfully by fusing AhSOS2 with expression vector pET -28a(+).Then the recombinant was expressed in E.coli strain BL21(DE3).Inducing with 1.0 mmol/L IPTG, a 51 kD fusion protein was obtained .Its expression quantity improved gradually with the extension of induction time, and attained the highest level after inducing for 8 hours.