CAJ | 학술논문

为了在转化当代获得纯合的转基因株系，本试验对以小麦花药为受体的转基因方法进行了系统研究。结果表明，金粉粒度0．6μm、金粉浓度100μg/枪、轰击距离6 cm、轰击压力1300 psi为基因枪转化小麦花药的最佳参数组合。用含有5％DMSO的0．2％秋水仙碱溶液处理单倍体植株4 h，染色体加倍效率最高，达到100％；用含1％DMSO的溶液处理12 h加倍效率最低，为62．2％。 PCR结果显示所有T0代阳性植株在T1代都无分离，说明该方法得到的转基因株系在T0代即为纯合系。本研究成功建立了以花药为受体的小麦转基因技术体系，为小麦遗传改良提供了一种新的方法。
위료재전화당대획득순합적전기인주계，본시험대이소맥화약위수체적전기인방법진행료계통연구。결과표명，금분립도0．6μm、금분농도100μg/창、굉격거리6 cm、굉격압력1300 psi위기인창전화소맥화약적최가삼수조합。용함유5％DMSO적0．2％추수선감용액처리단배체식주4 h，염색체가배효솔최고，체도100％；용함1％DMSO적용액처리12 h가배효솔최저，위62．2％。 PCR결과현시소유T0대양성식주재T1대도무분리，설명해방법득도적전기인주계재T0대즉위순합계。본연구성공건립료이화약위수체적소맥전기인기술체계，위소맥유전개량제공료일충신적방법。
To obtain homozygous transgenic wheat within one generation , the parameters involved in bombardment and chromosome doubling were studied in details .The results indicated that the best parameter combination for anther bombardment were 0.6-μm particle size, 100 μg per shot, 6-cm bombardment dis-tance and 1 300-psi bombardment pressure .The highest doubling efficiency ( 100%) was obtained when haploid plants were treated with 0.2%colchicine solution containing 5%DMSO for 4 hours, and that contai-ning 1%DMSO had the lowest doubling efficiency (62.2%).Specific PCR amplification showed that all T 0 positive plants had no segregation in T 1 generation, which indicated that the homozygous transgenic lines were obtained within one generation .This study established a successful transgenic technology system using isolated wheat anthers as bombardment recipient , which provided an efficient approach for wheat genetic improvement .