CAJ | 학술논문

目的：通过对抑郁模型大鼠的快速眼动睡眠剥夺，观察大鼠强迫游泳不动时间及大鼠纹状体5-羟色胺、多巴胺和腺苷水平的变化，了解睡眠剥夺快速改善抑郁情绪的可能机制。方法将30只成年健康雄性大鼠分为正常对照组（A组）、抑郁模型组（B组）、抑郁模型＋睡眠剥夺组（C组），在建立慢性轻度不可预见性应激的抑郁模型后，采用小平台水环境法对大鼠进行72 h快速眼动睡眠剥夺，以强迫游泳实验检测大鼠的不动时间，采用高效液相色谱-荧光检测法测定3组大鼠纹状体5-羟色胺、多巴胺水平，采用高效液相色谱-紫外检测法测定3组大鼠纹状体腺苷水平。结果21 d慢性轻度不可预见性应激后，大鼠的强迫游泳不动时间显著延长（ P＜0．05），快眼动睡眠剥夺后大鼠强迫游泳不动时间显著缩短（P＜0．01）。A组、C组大鼠纹状体5-羟色胺及腺苷水平均显著高于B组（ P＜0．05或0．01），C组大鼠纹状体多巴胺水平显著高于A组、B组（P＜0．01）。结论快速眼动睡眠剥夺可以逆转大鼠的抑郁样行为，并提高大鼠纹状体5-羟色胺、多巴胺水平，腺苷可能参与了睡眠剥夺的抗抑郁过程。
목적：통과대억욱모형대서적쾌속안동수면박탈，관찰대서강박유영불동시간급대서문상체5-간색알、다파알화선감수평적변화，료해수면박탈쾌속개선억욱정서적가능궤제。방법장30지성년건강웅성대서분위정상대조조（A조）、억욱모형조（B조）、억욱모형＋수면박탈조（C조），재건립만성경도불가예견성응격적억욱모형후，채용소평태수배경법대대서진행72 h쾌속안동수면박탈，이강박유영실험검측대서적불동시간，채용고효액상색보-형광검측법측정3조대서문상체5-간색알、다파알수평，채용고효액상색보-자외검측법측정3조대서문상체선감수평。결과21 d만성경도불가예견성응격후，대서적강박유영불동시간현저연장（ P＜0．05），쾌안동수면박탈후대서강박유영불동시간현저축단（P＜0．01）。A조、C조대서문상체5-간색알급선감수평균현저고우B조（ P＜0．05혹0．01），C조대서문상체다파알수평현저고우A조、B조（P＜0．01）。결론쾌속안동수면박탈가이역전대서적억욱양행위，병제고대서문상체5-간색알、다파알수평，선감가능삼여료수면박탈적항억욱과정。
Objective To observe rats’ motionless time during forced swimming and changes of 5-HT ,DA and adenosine in striatum via depriving REM sleep of depression-model rats and to find out possible mecha-nism of REM sleep deprivation improving emotional emotion .Methods Thirty healthy male adult rats were divided into normal control group (group A) ,depression-model (group B) and depression-model with sleep deprivation (group C) ,72-hour REM sleep deprivations were carried out using “flower pot” tech-nique after establishing chronic mild unpredictable stress depression-model ,motionless times detected u-sing forced swimming ,striatum 5-HT and DA levels measured with high efficiency liquid chromatography-fluorescence detection and adenosine with high efficiency liquid chromatography-ultraviolet .Results Mo-tionless time during forced swimming prolonged notably after 21-day chronic mild unpredictable stresses , (P<0 .05) and shortened after REM sleep deprivation (P<0 .01) .Striatum 5-HT and adenosine levels were significantly higher in both group A and C than B (P<0 .05 or 0 .01) ,so were the DA in group C than A and B (P<0 .01) .Conclusion REM sleep deprivation could reverse the depression-like behaviors of rats and increase 5-HT and DA levels in striatum ,adenosine may take part in the antidepressant process of REM sleep deprivation .