郑州大学学报(医学版)
鄭州大學學報(醫學版)
정주대학학보(의학판)
JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES)
2014年
1期
8-11
,共4页
冬凌草甲素%食管鳞状细胞癌%EC9706%增殖%凋亡
鼕凌草甲素%食管鱗狀細胞癌%EC9706%增殖%凋亡
동릉초갑소%식관린상세포암%EC9706%증식%조망
oridonin%esophageal squamous cell carcinoma%EC9706%proliferation%apoptosis
目的:观察冬凌草甲素对食管鳞状细胞癌EC9706细胞增殖、凋亡的影响。方法:用MTT法检测冬凌草甲素对EC9706细胞生长的抑制作用;采用流式细胞仪技术检测冬凌草甲素对EC9706细胞周期和细胞凋亡的影响,并计算凋亡率;激光共聚焦显微镜观察不同浓度冬凌草甲素作用后EC9706细胞中游离Ca2+荧光强度的变化。结果:MTT法显示冬凌草甲素作用EC9706细胞株后,抑制率随浓度增高而增高(F=370.600,P<0.001);流式细胞仪检测结果显示,冬凌草甲素作用EC9706细胞48 h后,G1/G0期细胞均显著增多(F=24.200,P<0.001);细胞凋亡结果显示,冬凌草甲素均能明显诱导EC9706细胞凋亡( P<0.05),冬凌草甲素40μmol/L组的作用效果最佳(F=10.214、37.820,P<0.001)。不同浓度冬凌草甲素均升高了EC9706细胞内游离Ca2+荧光强度(F=24.400, P<0.001)。结论:冬凌草甲素可抑制EC9706细胞增殖、促进细胞凋亡。
目的:觀察鼕凌草甲素對食管鱗狀細胞癌EC9706細胞增殖、凋亡的影響。方法:用MTT法檢測鼕凌草甲素對EC9706細胞生長的抑製作用;採用流式細胞儀技術檢測鼕凌草甲素對EC9706細胞週期和細胞凋亡的影響,併計算凋亡率;激光共聚焦顯微鏡觀察不同濃度鼕凌草甲素作用後EC9706細胞中遊離Ca2+熒光彊度的變化。結果:MTT法顯示鼕凌草甲素作用EC9706細胞株後,抑製率隨濃度增高而增高(F=370.600,P<0.001);流式細胞儀檢測結果顯示,鼕凌草甲素作用EC9706細胞48 h後,G1/G0期細胞均顯著增多(F=24.200,P<0.001);細胞凋亡結果顯示,鼕凌草甲素均能明顯誘導EC9706細胞凋亡( P<0.05),鼕凌草甲素40μmol/L組的作用效果最佳(F=10.214、37.820,P<0.001)。不同濃度鼕凌草甲素均升高瞭EC9706細胞內遊離Ca2+熒光彊度(F=24.400, P<0.001)。結論:鼕凌草甲素可抑製EC9706細胞增殖、促進細胞凋亡。
목적:관찰동릉초갑소대식관린상세포암EC9706세포증식、조망적영향。방법:용MTT법검측동릉초갑소대EC9706세포생장적억제작용;채용류식세포의기술검측동릉초갑소대EC9706세포주기화세포조망적영향,병계산조망솔;격광공취초현미경관찰불동농도동릉초갑소작용후EC9706세포중유리Ca2+형광강도적변화。결과:MTT법현시동릉초갑소작용EC9706세포주후,억제솔수농도증고이증고(F=370.600,P<0.001);류식세포의검측결과현시,동릉초갑소작용EC9706세포48 h후,G1/G0기세포균현저증다(F=24.200,P<0.001);세포조망결과현시,동릉초갑소균능명현유도EC9706세포조망( P<0.05),동릉초갑소40μmol/L조적작용효과최가(F=10.214、37.820,P<0.001)。불동농도동릉초갑소균승고료EC9706세포내유리Ca2+형광강도(F=24.400, P<0.001)。결론:동릉초갑소가억제EC9706세포증식、촉진세포조망。
Aim:To study the influence of oridonin on esophageal squamous cell carcinoma EC 9706 cell proliferation and apoptosis in vitro .Methods:MTT assay was used to detect the inhibition effect of oridonin on the growth of EC 9706;flow cytometry was applied to detect the impact of oridonin on cell cycle and apoptosis of EC 9706 , and calculated the apop-tosis rate of cells;change of Ca 2+fluorescence intensity of EC 9706 affected by different concentrations of oridonin was ob-served by laser scanning confocal microscope .Results:MTT assay showed that the inhibition rate in the experiment group increased with the rising of concentration (F=370.600,P<0.001) after oridonin acting on EC9706; results of cell cycle detected by flow cytometry showed that the number of cell in G 0 and G1 stage increased notably after oridonin acting on EC9706 for 48 hour respectively(F=24.200,P<0.001);results of apoptosis showed that oridonin could obviously induce the apoptosis of EC9706(P <0.05), and the effect was the best when the oridonin concentration was 40 μmol/L(F =10.214,37.820,P <0.001).Different concentrations of oridonin could increase Ca 2 +fluorescence intensity of EC9706,and the difference was significant (F =24.400,P <0.001).Conclusion: oridonin can improve the inhibition effects onproliferation and apoptosis of EC9706 cell.