CAJ | 학술논문

目的：观察沉默谷胱甘肽S转移酶-π（ GST-π）基因的表达对食管鳞状细胞癌顺铂耐药细胞系EC9706/cDDP细胞耐药性的影响。方法：构建靶向GST-π基因的siRNA重组慢病毒GSTs1i、 GSTsi2和无义对照GSTsiC，转染EC9706/cDDP细胞。通过RT-PCR及Western blot法检测GSTsi1、GSTsi2和GSTsiC感染前后EC9706/cDDP细胞中GST-πmRNA和蛋白的表达；MTT法检测感染GSTsi2、GSTsiC与未感染的EC9706/cDDP细胞对顺铂敏感性的变化。结果：感染GSTsi1、GSTsi2后EC9706/cDDP细胞GST-πmRNA的表达下调（F＝3．490，P＜0．001），其蛋白表达也减弱。感染GSTsi2的EC9706/cDDP细胞对顺铂的耐药指数较感染GSTsiC和未感染的EC9706/cDDP细胞降低（F＝50．510，P＜0．001）。结论：沉默GST-π基因的表达可降低EC9706/cDDP细胞的顺铂耐药性。
목적：관찰침묵곡광감태S전이매-π（ GST-π）기인적표체대식관린상세포암순박내약세포계EC9706/cDDP세포내약성적영향。방법：구건파향GST-π기인적siRNA중조만병독GSTs1i、 GSTsi2화무의대조GSTsiC，전염EC9706/cDDP세포。통과RT-PCR급Western blot법검측GSTsi1、GSTsi2화GSTsiC감염전후EC9706/cDDP세포중GST-πmRNA화단백적표체；MTT법검측감염GSTsi2、GSTsiC여미감염적EC9706/cDDP세포대순박민감성적변화。결과：감염GSTsi1、GSTsi2후EC9706/cDDP세포GST-πmRNA적표체하조（F＝3．490，P＜0．001），기단백표체야감약。감염GSTsi2적EC9706/cDDP세포대순박적내약지수교감염GSTsiC화미감염적EC9706/cDDP세포강저（F＝50．510，P＜0．001）。결론：침묵GST-π기인적표체가강저EC9706/cDDP세포적순박내약성。
Aim:To observe the effects of silencing the expression of GST-πon the drug-resistance of esophageal car-cinoma cells.Methods:The lentivirus of siRNA targeting to GST-πwas constructed.EC9706/cDDP cells were infected by the lentivirus named GSTsi1,GSTsi2.The mRNA and protein expressions of GST-πbefore and after infection were measured by RT-PCR and Western blot.Cells were divided into four groups:cells infected with GSTsi2, GSTsiC, untreat-ed EC9706/cDDP and EC9706 .Drug sensitivity to cDDP was determined by MTT .Results:The expression level of GST-πmRNA in cells infected by GSTsi1 and GSTsi2 were lower than those in control cells (F=3.490,P<0.001),and the ex-pression of GST-πprotein was also decreased .Compared with untreated EC9706/cDDP and EC9706/cDDP infected by GSTsiC,the resistance index of EC9706/cDDP infected by GSTsi2 decreased(F=50.510,P<0.001).Conclusion: Si-lencing the expression of GST-πby RNAi can partly reverse the drug resistance of EC 9706/cDDP.