中国医学创新
中國醫學創新
중국의학창신
MEDICAL INNOVATION OF CHINA
2014年
2期
121-122
,共2页
p73基因%急性淋巴细胞白血病
p73基因%急性淋巴細胞白血病
p73기인%급성림파세포백혈병
p73 Gene%Lymphoblastic leukemia
目的：研究p73基因在急性淋巴细胞性白血病（ALL）发病机制中的作用及临床意义。方法：收集32例ALL患者及30例正常对照组骨髓样本，DNA和RNA抽提后用RT-PCR检测p73基因的表达、甲基化特异性PCR（MSP）检测p73基因第一外显子甲基化状态，并结合临床资料进行分析。结果：11例p73基因阴性表达，阴性表达率为34.38%，其中9例第一外显子区域均发生甲基化（81.82%）；21例p73基因阳性表达，32例ALL患者中，仅1例发生甲基化（4.76%）；正常对照组30例，均为p73基因阳性表达，阳性表达率为100%。p73基因的阴性表达与ALL患者的高年龄（≥60岁）、高白细胞数（≥30×109/L）及对治疗时间延长（>4周取得完全缓解）有关。结论：p73基因异常表达与成人ALL的发病机制有关，具有一定的诊断及判断预后的价值；其甲基化可能是p73基因在ALL中表达沉默的主要机制。
目的：研究p73基因在急性淋巴細胞性白血病（ALL）髮病機製中的作用及臨床意義。方法：收集32例ALL患者及30例正常對照組骨髓樣本，DNA和RNA抽提後用RT-PCR檢測p73基因的錶達、甲基化特異性PCR（MSP）檢測p73基因第一外顯子甲基化狀態，併結閤臨床資料進行分析。結果：11例p73基因陰性錶達，陰性錶達率為34.38%，其中9例第一外顯子區域均髮生甲基化（81.82%）；21例p73基因暘性錶達，32例ALL患者中，僅1例髮生甲基化（4.76%）；正常對照組30例，均為p73基因暘性錶達，暘性錶達率為100%。p73基因的陰性錶達與ALL患者的高年齡（≥60歲）、高白細胞數（≥30×109/L）及對治療時間延長（>4週取得完全緩解）有關。結論：p73基因異常錶達與成人ALL的髮病機製有關，具有一定的診斷及判斷預後的價值；其甲基化可能是p73基因在ALL中錶達沉默的主要機製。
목적：연구p73기인재급성림파세포성백혈병（ALL）발병궤제중적작용급림상의의。방법：수집32례ALL환자급30례정상대조조골수양본，DNA화RNA추제후용RT-PCR검측p73기인적표체、갑기화특이성PCR（MSP）검측p73기인제일외현자갑기화상태，병결합림상자료진행분석。결과：11례p73기인음성표체，음성표체솔위34.38%，기중9례제일외현자구역균발생갑기화（81.82%）；21례p73기인양성표체，32례ALL환자중，부1례발생갑기화（4.76%）；정상대조조30례，균위p73기인양성표체，양성표체솔위100%。p73기인적음성표체여ALL환자적고년령（≥60세）、고백세포수（≥30×109/L）급대치료시간연장（>4주취득완전완해）유관。결론：p73기인이상표체여성인ALL적발병궤제유관，구유일정적진단급판단예후적개치；기갑기화가능시p73기인재ALL중표체침묵적주요궤제。
Objective:To investigate the effect of p73 gene on pathogenesis of acute lymphoblastic leukemia(ALL)and clinical significance of abnormal expression of p73 gene. Method:Genomic DNA and total RNA were extracted from 32 ALL and 30 normal bone marrow samples. The expression of p73 was evaluated by RT-PCR. The methylation specific PCR(MSP)was performed to examine the methylation status of p73 gene exon. Result:Of 32 ALL samples,11 showed without expression of p73 mRNA,with a negative rate of 34.38%. Normal samples all had expression of p73 gene,with a positive rate of 100%. 9 patients without expression of p73 were found to have methylation of p73 gene,whereas only one of patients with expression of p73 mRNA was found to have methylation. Negative expression of p73 gene was associated with higher age(≥60 years old ),higher leucocyte count(≥30×109/L) and delayed reaction to therapy(patients attained complete remission after 4 weeks). Conclusion:Negative expression of p73 gene probably plays a role in the diagnosis and pathogenesis of ALL,and might have clinical significance in predicting its prognosis. Methylation may be the major mechanism of gene silence of p73 in ALL.
