中国医学创新
中國醫學創新
중국의학창신
MEDICAL INNOVATION OF CHINA
2014年
2期
11-12
,共2页
牙周炎发生%CD4+CD25+T细胞%免疫调节
牙週炎髮生%CD4+CD25+T細胞%免疫調節
아주염발생%CD4+CD25+T세포%면역조절
Periodontal lesion%CD4+CD25+Treg%Local immune
目的：探讨CD4+CD25+Treg在牙周炎发生时的免疫调节作用。方法：选择35只成年SPF级Wistar大鼠，丝线结扎加高糖饮食建立大鼠牙周炎发生的动物模型，获得类似牙周炎自然病程的连续组织标本，病理切片确定病损，分别获取第4、8、12、16周的大鼠外周血，术前未结扎组对照。以流式细胞仪检测不同时段外周血中CD4+CD25+Treg的比例变化。结果：建立了Wistar大鼠牙周炎发生的动物模型，病理切片确定了牙周病损的存在。结果显示伴随牙周炎发生的牙槽骨的吸收逐渐增多。流式细胞仪结果显示结扎后第4、8、12、16周外周血中CD4+CD25+Treg占CD4+T细胞的比例为7.70%、9.12%、9.74%、10.31%，与术前比较差异均有统计学意义（P<0.05）。结论：外周血中CD4+CD25+Treg自牙周炎发生早期逐渐升高，牙周炎发生发展规律一致，从而证实CD4+CD25+Treg细胞参与介导了牙周炎发生的免疫调控。
目的：探討CD4+CD25+Treg在牙週炎髮生時的免疫調節作用。方法：選擇35隻成年SPF級Wistar大鼠，絲線結扎加高糖飲食建立大鼠牙週炎髮生的動物模型，穫得類似牙週炎自然病程的連續組織標本，病理切片確定病損，分彆穫取第4、8、12、16週的大鼠外週血，術前未結扎組對照。以流式細胞儀檢測不同時段外週血中CD4+CD25+Treg的比例變化。結果：建立瞭Wistar大鼠牙週炎髮生的動物模型，病理切片確定瞭牙週病損的存在。結果顯示伴隨牙週炎髮生的牙槽骨的吸收逐漸增多。流式細胞儀結果顯示結扎後第4、8、12、16週外週血中CD4+CD25+Treg佔CD4+T細胞的比例為7.70%、9.12%、9.74%、10.31%，與術前比較差異均有統計學意義（P<0.05）。結論：外週血中CD4+CD25+Treg自牙週炎髮生早期逐漸升高，牙週炎髮生髮展規律一緻，從而證實CD4+CD25+Treg細胞參與介導瞭牙週炎髮生的免疫調控。
목적：탐토CD4+CD25+Treg재아주염발생시적면역조절작용。방법：선택35지성년SPF급Wistar대서，사선결찰가고당음식건립대서아주염발생적동물모형，획득유사아주염자연병정적련속조직표본，병리절편학정병손，분별획취제4、8、12、16주적대서외주혈，술전미결찰조대조。이류식세포의검측불동시단외주혈중CD4+CD25+Treg적비례변화。결과：건립료Wistar대서아주염발생적동물모형，병리절편학정료아주병손적존재。결과현시반수아주염발생적아조골적흡수축점증다。류식세포의결과현시결찰후제4、8、12、16주외주혈중CD4+CD25+Treg점CD4+T세포적비례위7.70%、9.12%、9.74%、10.31%，여술전비교차이균유통계학의의（P<0.05）。결론：외주혈중CD4+CD25+Treg자아주염발생조기축점승고，아주염발생발전규률일치，종이증실CD4+CD25+Treg세포삼여개도료아주염발생적면역조공。
Objective:To explore CD4+CD25+T cells in peripheral blood during the progression of experimental periodontitis. Method:35 adult SPF Wistar rats were selected during rat model of periodontitis occurs by means of high-sugar diet and silk ligature. Histological sections and peripheral blood of periodontitis tissue samples were obtained respectively at 4,8,12,16 weeks. Non-ligation group as control. The frequency of CD4+CD25+T cells in peripheral blood during the progression of experimental periodontitis was measured by fluorescence-activated cell sorter(FACS)method. Result:Wistar rat model was established to determine the existence of histology periodontal loss. The results showed that the absorption of alveolar bone associated with periodontitis occurs gradually increased. Flow cytometry showed that the frequency of CD4+CD25+T cells accounted for 7.70%,9.12%,9.74%, 10.31%at the 4,8,12,16 weeks after ligation compared with control group were statistically significant(P<0.05).Conclusion:During the progression of experimental periodontitis,the percentage of CD4+CD25+Treg increase in peripheral blood. CD4+CD25+Treg cells may play regulatory roles in the progression.
