中国老年学杂志
中國老年學雜誌
중국노년학잡지
CHINESE JOURNAL OF GERONTOLOGY
2014年
8期
2199-2201
,共3页
张可可%连晓怡%郑金旭%端礼荣
張可可%連曉怡%鄭金旭%耑禮榮
장가가%련효이%정금욱%단례영
氨基胍%肺纤维化%一氧化氮%转化生长因子β1
氨基胍%肺纖維化%一氧化氮%轉化生長因子β1
안기고%폐섬유화%일양화담%전화생장인자β1
Aminoguandine%Pulmonary Fibrosis%Nitric oxide%Transforming growth factor β1
目的:探讨氨基胍( AG)对博莱霉素( BLM)诱导SD大鼠肺纤维化中的干预作用。方法将116只 SD大鼠随机分成5组:正常对照组、BLM组、AG组、激素组和AG+激素组。经气道给予BLM或生理盐水后,每天给不同组别相应的药物或生理盐水连续28 d。分别在第7,14,21,28天随机处死各组老鼠5只。处死前,取肺泡灌洗液用硝酸还原酶法测一氧化氮( NO )的表达。用碱水解法测肺组织中羟脯氨酸的含量及用 RT-PCR检测肺组织中转化生长因子( TGF)-β1 mRNA的相对表达量。结果①与BLM组比较,AG组、激素组和AG+激素组支气管肺泡灌洗液( BALF)中NO的表达在第7,14天下降(P<0.05),肺组织羟脯氨酸含量减少(P<0.05)。②各治疗组肺组织TGF-β1 mRNA的相对表达量和 BLM组比较在7,14,21 d明显增加( P<0.05)。结论 AG可以降低BLM诱导的肺纤维化程度,作用机制可能是通过抑制TGF-β1的基因表达。
目的:探討氨基胍( AG)對博萊黴素( BLM)誘導SD大鼠肺纖維化中的榦預作用。方法將116隻 SD大鼠隨機分成5組:正常對照組、BLM組、AG組、激素組和AG+激素組。經氣道給予BLM或生理鹽水後,每天給不同組彆相應的藥物或生理鹽水連續28 d。分彆在第7,14,21,28天隨機處死各組老鼠5隻。處死前,取肺泡灌洗液用硝痠還原酶法測一氧化氮( NO )的錶達。用堿水解法測肺組織中羥脯氨痠的含量及用 RT-PCR檢測肺組織中轉化生長因子( TGF)-β1 mRNA的相對錶達量。結果①與BLM組比較,AG組、激素組和AG+激素組支氣管肺泡灌洗液( BALF)中NO的錶達在第7,14天下降(P<0.05),肺組織羥脯氨痠含量減少(P<0.05)。②各治療組肺組織TGF-β1 mRNA的相對錶達量和 BLM組比較在7,14,21 d明顯增加( P<0.05)。結論 AG可以降低BLM誘導的肺纖維化程度,作用機製可能是通過抑製TGF-β1的基因錶達。
목적:탐토안기고( AG)대박래매소( BLM)유도SD대서폐섬유화중적간예작용。방법장116지 SD대서수궤분성5조:정상대조조、BLM조、AG조、격소조화AG+격소조。경기도급여BLM혹생리염수후,매천급불동조별상응적약물혹생리염수련속28 d。분별재제7,14,21,28천수궤처사각조로서5지。처사전,취폐포관세액용초산환원매법측일양화담( NO )적표체。용감수해법측폐조직중간포안산적함량급용 RT-PCR검측폐조직중전화생장인자( TGF)-β1 mRNA적상대표체량。결과①여BLM조비교,AG조、격소조화AG+격소조지기관폐포관세액( BALF)중NO적표체재제7,14천하강(P<0.05),폐조직간포안산함량감소(P<0.05)。②각치료조폐조직TGF-β1 mRNA적상대표체량화 BLM조비교재7,14,21 d명현증가( P<0.05)。결론 AG가이강저BLM유도적폐섬유화정도,작용궤제가능시통과억제TGF-β1적기인표체。
Objective To investigate aminoguandine ( AG)′s role in bleomycin ( BLM)-induced pulmonary fibrosis in rats .Methods One hundred and sixteen rats were randomly divided into 5 groups, including normal control, BLM, AG, dexamethasone group, AG+dex-amethasone groups .After administration of BLM intratracheally , AG, dexamethasone were given in each group daily , according to the dosage designed.Rats were killed on the 7th, 14th, 21th, 28th days.The content of NO in the bronchoalveolar lavage fluid (BALF) was meas-ured.The degree of fibrosis was evaluated by pulmonary hydroxyproline assay , the expression of TGF-β1 mRNA in lung tissues by RT-PCR. Results (1)The expressions of NO in BALF in AG, dexamethasone, AG+dexamethasone groups were lower than those in BLM group on the 7th,14th days(P<0.05).The expressions of hydroxyproline in lung tissues were lower than those in BLM group on the 14th, 21th days (P<0.05).(2)The expressions of TGF-β1 mRNA in lung tissues were lower than those in BLM group on the 7th, 14th, 21th days(P<0.05 ) .Conclusions AG attenuates BLM-induced lung fibrosis , and it may be relate to the downregulation of TGF-β1 expression .