妇产与遗传（电子版）
婦產與遺傳（電子版）
부산여유전（전자판）
Obstetrics-Gynecology and Genetics(Electronic Edition)
2013年
4期
20-23
,共4页
辐射%卵巢肿瘤%蛋白激酶类
輻射%卵巢腫瘤%蛋白激酶類
복사%란소종류%단백격매류
Radiation%Ovarian neoplasms%Protein kinases
目的：研究辐射对转染DNA依赖的蛋白激酶shRNA-（DNA-PK）的卵巢癌Skov3细胞G2的影响。方法培养4个6孔板的Skov3细胞及设计shRNA和shNC载体（对照载体）后进行转染，转染成功后将其分为四个组：shNC组、DNA-PK shRNA组、shNC+照射组、DNA-PK shRNA+照射组，并对照射组分别进行辐射4 h及28 h后采集细胞，采用流式细胞术检测四组Skov3细胞进入G2期的情况。结果（1）辐射4 h后，四组进入G2期的Skov3细胞所占比例差异具有统计学差异（F=8.65，P<0.01），DNA-PK shRNA+照射组进入G2期的Skov3细胞比例为（18.56±2.79）%，与shNC+辐射组（18.56±1.57）%比较无差异（t=0.00，P=1.00），但明显高于DNA-PK shRNA转染组（14.38±1.70）%（t=3.13，P=0.01）；（2）辐射28 h后DNA-PK shRNA+照射组进入G2期的Skov3细胞比例是（11.26±3.31）%，与转染shNC+辐射组（11.71±1.28）%比较无差异（t=0.31，P=0.76），但明显高于DNA-PK shRNA转染组（4.42±1.59）%（t=4.56，P<0.01）。结论通过干扰DNA-PK的表达，可使辐射后卵巢癌G2期细胞明显增多，从而提高卵巢癌细胞对于辐射的敏感性。
目的：研究輻射對轉染DNA依賴的蛋白激酶shRNA-（DNA-PK）的卵巢癌Skov3細胞G2的影響。方法培養4箇6孔闆的Skov3細胞及設計shRNA和shNC載體（對照載體）後進行轉染，轉染成功後將其分為四箇組：shNC組、DNA-PK shRNA組、shNC+照射組、DNA-PK shRNA+照射組，併對照射組分彆進行輻射4 h及28 h後採集細胞，採用流式細胞術檢測四組Skov3細胞進入G2期的情況。結果（1）輻射4 h後，四組進入G2期的Skov3細胞所佔比例差異具有統計學差異（F=8.65，P<0.01），DNA-PK shRNA+照射組進入G2期的Skov3細胞比例為（18.56±2.79）%，與shNC+輻射組（18.56±1.57）%比較無差異（t=0.00，P=1.00），但明顯高于DNA-PK shRNA轉染組（14.38±1.70）%（t=3.13，P=0.01）；（2）輻射28 h後DNA-PK shRNA+照射組進入G2期的Skov3細胞比例是（11.26±3.31）%，與轉染shNC+輻射組（11.71±1.28）%比較無差異（t=0.31，P=0.76），但明顯高于DNA-PK shRNA轉染組（4.42±1.59）%（t=4.56，P<0.01）。結論通過榦擾DNA-PK的錶達，可使輻射後卵巢癌G2期細胞明顯增多，從而提高卵巢癌細胞對于輻射的敏感性。
목적：연구복사대전염DNA의뢰적단백격매shRNA-（DNA-PK）적란소암Skov3세포G2적영향。방법배양4개6공판적Skov3세포급설계shRNA화shNC재체（대조재체）후진행전염，전염성공후장기분위사개조：shNC조、DNA-PK shRNA조、shNC+조사조、DNA-PK shRNA+조사조，병대조사조분별진행복사4 h급28 h후채집세포，채용류식세포술검측사조Skov3세포진입G2기적정황。결과（1）복사4 h후，사조진입G2기적Skov3세포소점비례차이구유통계학차이（F=8.65，P<0.01），DNA-PK shRNA+조사조진입G2기적Skov3세포비례위（18.56±2.79）%，여shNC+복사조（18.56±1.57）%비교무차이（t=0.00，P=1.00），단명현고우DNA-PK shRNA전염조（14.38±1.70）%（t=3.13，P=0.01）；（2）복사28 h후DNA-PK shRNA+조사조진입G2기적Skov3세포비례시（11.26±3.31）%，여전염shNC+복사조（11.71±1.28）%비교무차이（t=0.31，P=0.76），단명현고우DNA-PK shRNA전염조（4.42±1.59）%（t=4.56，P<0.01）。결론통과간우DNA-PK적표체，가사복사후란소암G2기세포명현증다，종이제고란소암세포대우복사적민감성。
Objective To investigate the impact on the Skov3 cell of ovarian cancer transfected DNA-dependent protein kinase shRNA in G2 phase after radiation. Methods 4 six-well plates were chosen to culture Skov3 cells and transfected to shRNA and shNC carrier after designing. Then the 4 six-well plates were divided into 4 groups:shNC、DNA-PK shRNA、shNC+radiation and DNA-PK shRNA+radiation. The cells in group shNC+radiation and DNA-PK shRNA+radiation were collected after 4h and 28h radiation. All the Skov3 cells were detected to confirm the proportion of G2 phase though flow cytometry. Results (1) There was significant difference of the proportion of G2 phase in Skov3 cells among the 4 groups after 4h radiation. The proportion of G2 phase of Skov3 cells in group (DNA-PK shRNA+radiation) is (18.56±2.79)%, which was no difference with the group (shNC+radiation) (18.56 ± 1.57)%(t=0.00, P=1.00),but higher than group (DNA-PK shRNA) (14.38 ± 1.70)%(t=3.13,P=0.01);(2)The proportion of Skov3 cells in G2 phase after 28h radiation is(11.26±3.31)%, There was no significant difference with group (shNC+radiation) (t=0.31, P=0.76). But it was higher than the group (DNA-PK shRNA) (4.42±1.59)%(t=4.56, P<0.01). Conclusions It can increased the G2 phase cells of ovarian cancer after radiation significantly though interfere the expression of DNA-PK, so that it can increase the sensitivity of cells in ovarian cancer to radiation.
