农产品加工·学刊(中)
農產品加工·學刊(中)
농산품가공·학간(중)
AEM RODUCTS ROCESSING
2014年
3期
5-8
,共4页
四棱豆%碱性蛋白酶%酶解%水解度
四稜豆%堿性蛋白酶%酶解%水解度
사릉두%감성단백매%매해%수해도
winged bean%alkaline protease%enzymatic hydrolysis%hydrolysis degree
以四棱豆的种子为原料,以水解度为衡量指标,通过单因素试验和正交试验分析,研究碱性蛋白酶Alcalase水解四棱豆蛋白质的最佳水解条件。结果表明,底物质量分数为3%, pH值为8.0,温度为60℃,水解时间为5 h,[E]/[S]为6%,在此条件下四棱豆蛋白质水解度为22.95%。
以四稜豆的種子為原料,以水解度為衡量指標,通過單因素試驗和正交試驗分析,研究堿性蛋白酶Alcalase水解四稜豆蛋白質的最佳水解條件。結果錶明,底物質量分數為3%, pH值為8.0,溫度為60℃,水解時間為5 h,[E]/[S]為6%,在此條件下四稜豆蛋白質水解度為22.95%。
이사릉두적충자위원료,이수해도위형량지표,통과단인소시험화정교시험분석,연구감성단백매Alcalase수해사릉두단백질적최가수해조건。결과표명,저물질량분수위3%, pH치위8.0,온도위60℃,수해시간위5 h,[E]/[S]위6%,재차조건하사릉두단백질수해도위22.95%。
This paper, with the seeds of winged bean as raw materials and the hydrolysis degree as measuring indicator, studies the optimal technological parameters of alkaline protease hydrolysis of proteins in the winged bean seeds by single factor experiment and orthogonal array design. Results show that a maximum hydrolysis degree of winged bean proteins of 22.95% is obtained through the optimal hydrolysis procedure conducted for 5 h with a substrate concentration of 3%and an [E]/[S] ratio of 6%at 60℃and pH 8.0.