中国动物传染病学报
中國動物傳染病學報
중국동물전염병학보
CHINESE JOURNAL OF VETERINARY PARASITOLOGY
2014年
2期
39-45
,共7页
姜一峰%周艳君%童武%虞凌雪%程群%杨莘%夏天奇%李丽薇%高飞%童光志
薑一峰%週豔君%童武%虞凌雪%程群%楊莘%夏天奇%李麗薇%高飛%童光誌
강일봉%주염군%동무%우릉설%정군%양신%하천기%리려미%고비%동광지
猪繁殖与呼吸综合征病毒%基因工程双标记疫苗%最小免疫剂量
豬繁殖與呼吸綜閤徵病毒%基因工程雙標記疫苗%最小免疫劑量
저번식여호흡종합정병독%기인공정쌍표기역묘%최소면역제량
Porcine reproductive and respiratory syndrome virus%dual-marker vaccine%minimum immunization dose
将35头40日龄猪繁殖与呼吸综合征病毒（Porcine reproductive and respiratory syndrome virus, PRRSV）、猪瘟病毒（Classical swine fever virus，CSFV）、猪圆环病毒（Porcine circovirus type 2，PCV2）抗原及抗体均为阴性的健康仔猪，随机分成7组，每组5头，命名为XJ1~XJ7组。XJ1~XJ6组分别免疫PRRSV基因工程双标记疫苗rHN4-△25+NP49株，接种剂量分别为102TCID50/头、103TCID50/头、104TCID50/头、105TCID50/头、106TCID50/头和107TCID50/头，XJ7组为对照组，按1mL/头剂量接种DMEM。各试验组在免疫后28 d，用3×104.0TCID50/头剂量感染HP-PRRSV HuN4第5代强毒，疫苗免疫和攻毒后观察和检测各组试验猪的免疫效果。结果表明：攻毒前XJ2-XJ6组PRRSV ELISA抗体在免疫后14 d全部转为阳性，XJ1组在免疫后21 d转为阳性，但维持在较低水平；XJ2-XJ6组针对标记基因NP49的特异性抗体在免疫后28 d转阳性。攻毒后对照组全部发病，且死亡2头，XJ1有4头发病，XJ2-XJ6组无发病和死亡，本研究结果表明PRRSV基因工程双标记疫苗rHN4-△25+NP49株对仔猪提供免疫保护的最小免疫剂量为103TCID50/头。
將35頭40日齡豬繁殖與呼吸綜閤徵病毒（Porcine reproductive and respiratory syndrome virus, PRRSV）、豬瘟病毒（Classical swine fever virus，CSFV）、豬圓環病毒（Porcine circovirus type 2，PCV2）抗原及抗體均為陰性的健康仔豬，隨機分成7組，每組5頭，命名為XJ1~XJ7組。XJ1~XJ6組分彆免疫PRRSV基因工程雙標記疫苗rHN4-△25+NP49株，接種劑量分彆為102TCID50/頭、103TCID50/頭、104TCID50/頭、105TCID50/頭、106TCID50/頭和107TCID50/頭，XJ7組為對照組，按1mL/頭劑量接種DMEM。各試驗組在免疫後28 d，用3×104.0TCID50/頭劑量感染HP-PRRSV HuN4第5代彊毒，疫苗免疫和攻毒後觀察和檢測各組試驗豬的免疫效果。結果錶明：攻毒前XJ2-XJ6組PRRSV ELISA抗體在免疫後14 d全部轉為暘性，XJ1組在免疫後21 d轉為暘性，但維持在較低水平；XJ2-XJ6組針對標記基因NP49的特異性抗體在免疫後28 d轉暘性。攻毒後對照組全部髮病，且死亡2頭，XJ1有4頭髮病，XJ2-XJ6組無髮病和死亡，本研究結果錶明PRRSV基因工程雙標記疫苗rHN4-△25+NP49株對仔豬提供免疫保護的最小免疫劑量為103TCID50/頭。
장35두40일령저번식여호흡종합정병독（Porcine reproductive and respiratory syndrome virus, PRRSV）、저온병독（Classical swine fever virus，CSFV）、저원배병독（Porcine circovirus type 2，PCV2）항원급항체균위음성적건강자저，수궤분성7조，매조5두，명명위XJ1~XJ7조。XJ1~XJ6조분별면역PRRSV기인공정쌍표기역묘rHN4-△25+NP49주，접충제량분별위102TCID50/두、103TCID50/두、104TCID50/두、105TCID50/두、106TCID50/두화107TCID50/두，XJ7조위대조조，안1mL/두제량접충DMEM。각시험조재면역후28 d，용3×104.0TCID50/두제량감염HP-PRRSV HuN4제5대강독，역묘면역화공독후관찰화검측각조시험저적면역효과。결과표명：공독전XJ2-XJ6조PRRSV ELISA항체재면역후14 d전부전위양성，XJ1조재면역후21 d전위양성，단유지재교저수평；XJ2-XJ6조침대표기기인NP49적특이성항체재면역후28 d전양성。공독후대조조전부발병，차사망2두，XJ1유4두발병，XJ2-XJ6조무발병화사망，본연구결과표명PRRSV기인공정쌍표기역묘rHN4-△25+NP49주대자저제공면역보호적최소면역제량위103TCID50/두。
Thirty five 40-days old piglets without Porcine reproductive and respiratory syndrome virus (PRRSV), Porcine circovirus (PCV2) and Classical swine fever virus (CSFV) were selected and randomly divided into 7 groups (XJ1-XJ7). Each of piglets in groups XJ1-XJ6 was inoculated with 102TCID50 to 107TCID50 of HP-PRRS dual-marker vaccine rHN4-Δ25+NP49 strain, respectively. Group XJ7 served as infection control. On 28 day post immunization, all animal were infected with 3×104TCID50 HuN4-F5 strain. Fever and other clinical signs were recorded daily. Pigs in group XJ2-XJ6 developed high level of humoral response against PRRSV from day 14 post immunization while piglets in group XJ1 developed a low level PRRSV antibody response from day 21 post immunization. Four piglets in group XJ1 and 5 piglets in XJ7 had various disease manifestations, including persistently high fever and anorexia. Two piglets in group XJ7 died at days 5 and 20 post challenge. On the contrast, piglets in groups XJ2-XJ6 did not show any clinical signs post challenge. These results indicated that the minimum immunization dose of rHN4-Δ25+NP49 strain was 103TCID50, which conferred effective protection against HP-PRRSV.
