CAJ | 학술논문

本文主要对细胞的泛素-蛋白酶体系统是否参与新城疫病毒（Newcastle disease virus，NDV）的复制过程进行了研究。用NDV感染HeLa细胞，对细胞样品进行Western blot实验，并检测细胞26S蛋白酶体的三种蛋白水解活性变化。同时使用多种泛素-蛋白酶体系统相关的抑制剂处理细胞并做NDV感染，测定细胞上清中病毒TCID50值，比较药物处理与DMSO处理对病毒增殖的影响。结果表明，HeLa细胞在感染NDV后，细胞内泛素化蛋白水平降低，而26S蛋白酶体的三种蛋白水解活性都显著升高；使用泛素-蛋白酶体系统抑制剂后NDV的增殖受到了明显的抑制，证明NDV在HeLa细胞内的增殖与细胞自身的泛素-蛋白酶体系统关系密切。
본문주요대세포적범소-단백매체계통시부삼여신성역병독（Newcastle disease virus，NDV）적복제과정진행료연구。용NDV감염HeLa세포，대세포양품진행Western blot실험，병검측세포26S단백매체적삼충단백수해활성변화。동시사용다충범소-단백매체계통상관적억제제처리세포병주NDV감염，측정세포상청중병독TCID50치，비교약물처리여DMSO처리대병독증식적영향。결과표명，HeLa세포재감염NDV후，세포내범소화단백수평강저，이26S단백매체적삼충단백수해활성도현저승고；사용범소-단백매체계통억제제후NDV적증식수도료명현적억제，증명NDV재HeLa세포내적증식여세포자신적범소-단백매체계통관계밀절。
The objective of the present study was to examine the effect of cellular ubiquitin-proteasome system on Newcastle disease virus (NDV) replication in Hela cells. To determine whether ubiquitin-proteasome system was activated in NDV infected HeLa cells, cellular samples were taken at indicated times for Western blot and for measurement of chymotrypsin-, trypsin-and caspase-like activities. Additionally, NDV infected HeLa cells were treated with different inhibitors of ubiquitin-proteasome system and virus titers (TCID50) in culture supernatants were determined. A significant increase in 26S proteasome activity was observed after NDV infection while ubiquitinated proteins decreased. The application of inhibitors significantly reduced NDV production and delayed viral propagation. In conclusion, ubiquitin-proteasome system plays a critical role in NDV replication in HeLa cells.