CAJ | 학술논문

目的：观察雏菊叶龙胆是否对坐骨神经损伤后有促进功能恢复的作用,其中是否有睫状神经营养因子的参与。方法雄性Wistar大鼠225只,右侧坐骨神经离断后显微镜下缝合。造模后随机分为对照组、雏菊叶龙胆25 mg组、50 mg组、75 mg组和甲钴胺组5组。对照组生理盐水注射,其他组相应剂量的雏菊叶龙胆及甲钴胺注射,1、3、5周测定神经传导速度及腓肠肌肌细胞截面积,免疫组化分析睫状神经营养因子( CNTF)阳性细胞比率。结果雏菊叶龙胆50 mg、75 mg组与对照组、甲钴胺组神经传导速度相比有明显差异,雏菊叶龙胆25 mg组与75 mg、50 mg组相比有明显差异(P=0.025)。3周时对照组、甲钴胺组、雏菊叶龙胆25 mg、50 mg、75 mg组腓肠肌肌细胞截面积分别为：(455.06±29.38)μm2、(679.03±81.48)μm2、(465.31±71.55)μm2、(670.24±91.26)μm2、(669.28±78.54)μm2,对照组、雏菊叶龙胆25 mg组与其他组比较有显著差异(P<0.05)。3周时各组睫状神经营养因子免疫组化可见雏菊叶龙胆25 mg组和对照组与雏菊叶龙胆50 mg、75 mg组比较结果有统计学差异。结论雏菊叶龙胆可促进坐骨神经损伤后功能恢复,可促进神经功能恢复中有睫状神经营养因子参与。
목적：관찰추국협룡담시부대좌골신경손상후유촉진공능회복적작용,기중시부유첩상신경영양인자적삼여。방법웅성Wistar대서225지,우측좌골신경리단후현미경하봉합。조모후수궤분위대조조、추국협룡담25 mg조、50 mg조、75 mg조화갑고알조5조。대조조생리염수주사,기타조상응제량적추국협룡담급갑고알주사,1、3、5주측정신경전도속도급비장기기세포절면적,면역조화분석첩상신경영양인자( CNTF)양성세포비솔。결과추국협룡담50 mg、75 mg조여대조조、갑고알조신경전도속도상비유명현차이,추국협룡담25 mg조여75 mg、50 mg조상비유명현차이(P=0.025)。3주시대조조、갑고알조、추국협룡담25 mg、50 mg、75 mg조비장기기세포절면적분별위：(455.06±29.38)μm2、(679.03±81.48)μm2、(465.31±71.55)μm2、(670.24±91.26)μm2、(669.28±78.54)μm2,대조조、추국협룡담25 mg조여기타조비교유현저차이(P<0.05)。3주시각조첩상신경영양인자면역조화가견추국협룡담25 mg조화대조조여추국협룡담50 mg、75 mg조비교결과유통계학차이。결론추국협룡담가촉진좌골신경손상후공능회복,가촉진신경공능회복중유첩상신경영양인자삼여。
Objective To study the rehabilitation effect of Bellidifolin for injuried sciatic nerve,and to explore whether ciliary neurotro-phic factor ( CNTF) is involved in this mechanism. Methods The right sciatic nerver of 225 male wistar rats was cut and sewed under mi-croscopy. Rats were devided into 5 groups,as control group,Bellidifolin 25 mg group,50 mg group、75 mg group and Mecobalamin group. The control group were injected sodium chloride,other groups were injected different dose of Bellidifolin and Mecobalamin. 1,3 and 5 weeks later, the motor nerve conduction velocity( MNVC) and gastrocnemius muscle cross-sectional area were detected,CNTF positive area were analysed by immunohistochemical method. Results There were differences among bellidifolin groups,control group and mecobalamin group in Nerve conduction velocity. Within Bellidifolin groups,50 mg group compared with 25 mg and 75 mg groups,there were statistically differences( P=0. 025). Three weeks after operation,gastrocnemius muscle cross-sectional area of control group,mecobalaming grop and Bellidifolin 25 mg group,50 mg group,and 75 mg group were(455. 06 ± 29. 38),(679. 03 ± 81. 48),(465. 31 ± 71. 55),(670. 24 ± 91. 26) and (669. 28 ± 78. 54) respectively,compared with control group and Bellidifolin 25 mg group,others had a significant difference(P<0. 05). CNTF expres-sion showed billidifolin 50 mg group are higher than others(P<0. 05). Conclusion Bellidifolin can improve the rehabilitation of injured sciatic nerve. CNTF is involved in this mechnism.