全科医学临床与教育
全科醫學臨床與教育
전과의학림상여교육
CLINICAL EDUCATION OF GENERAL PRACTICE
2014年
2期
131-133
,共3页
章卫桥%张志杰%郑钧%邱永明
章衛橋%張誌傑%鄭鈞%邱永明
장위교%장지걸%정균%구영명
全脑缺血%缺血后处理%缺血再灌注%神经保护
全腦缺血%缺血後處理%缺血再灌註%神經保護
전뇌결혈%결혈후처리%결혈재관주%신경보호
global cerebral ischemia%ischemia-reperfusion injury%ischemic postconditioning%neuroprotection
目的：探讨缺血后处理对大鼠全脑缺血再灌注损伤的影响。方法30只雄性SD大鼠随机分为三组（n=10），建立四血管阻断全脑缺血模型。假手术组：单纯麻醉和手术操作，无缺血过程；缺血再灌注组：缺血10 min后再灌注；缺血后处理组：缺血10 min后以10 s再灌注/10 s阻断，共6个循环实现缺血后处理。再灌注后于第1天和第3天进行行为学观察；在72 h后进行病理学检测。结果 Morris水迷宫提示：再灌注后第1天，缺血再灌注组的平均上台时间（84.76±10.22）s较假手术组（27.08±7.52）s明显延长，差异有统计学意义（t=14.84， P＜0.05），缺血后处理组的平均上台时间（50.24±9.72）s明显少于缺血再灌注组，差异有统计学意义（t=7.74， P＜0.05）。再灌注后第3天，缺血再灌注组的上台时间（55.90±11.26）s仍明显长于缺血后处理组（29.22±5.82）s，差异有统计学意义（t=6.65， P＜0.05）。72 h后病理学染色提示：缺血再灌注组和缺血后处理组海马CA1区存活神经元分别为假手术组的25.90％和64.60％。结论缺血后处理可以明显减少大鼠全脑缺血再灌注后神经元死亡，减轻行为学缺损，对大鼠全脑缺血再灌注有神经保护作用。
目的：探討缺血後處理對大鼠全腦缺血再灌註損傷的影響。方法30隻雄性SD大鼠隨機分為三組（n=10），建立四血管阻斷全腦缺血模型。假手術組：單純痳醉和手術操作，無缺血過程；缺血再灌註組：缺血10 min後再灌註；缺血後處理組：缺血10 min後以10 s再灌註/10 s阻斷，共6箇循環實現缺血後處理。再灌註後于第1天和第3天進行行為學觀察；在72 h後進行病理學檢測。結果 Morris水迷宮提示：再灌註後第1天，缺血再灌註組的平均上檯時間（84.76±10.22）s較假手術組（27.08±7.52）s明顯延長，差異有統計學意義（t=14.84， P＜0.05），缺血後處理組的平均上檯時間（50.24±9.72）s明顯少于缺血再灌註組，差異有統計學意義（t=7.74， P＜0.05）。再灌註後第3天，缺血再灌註組的上檯時間（55.90±11.26）s仍明顯長于缺血後處理組（29.22±5.82）s，差異有統計學意義（t=6.65， P＜0.05）。72 h後病理學染色提示：缺血再灌註組和缺血後處理組海馬CA1區存活神經元分彆為假手術組的25.90％和64.60％。結論缺血後處理可以明顯減少大鼠全腦缺血再灌註後神經元死亡，減輕行為學缺損，對大鼠全腦缺血再灌註有神經保護作用。
목적：탐토결혈후처리대대서전뇌결혈재관주손상적영향。방법30지웅성SD대서수궤분위삼조（n=10），건립사혈관조단전뇌결혈모형。가수술조：단순마취화수술조작，무결혈과정；결혈재관주조：결혈10 min후재관주；결혈후처리조：결혈10 min후이10 s재관주/10 s조단，공6개순배실현결혈후처리。재관주후우제1천화제3천진행행위학관찰；재72 h후진행병이학검측。결과 Morris수미궁제시：재관주후제1천，결혈재관주조적평균상태시간（84.76±10.22）s교가수술조（27.08±7.52）s명현연장，차이유통계학의의（t=14.84， P＜0.05），결혈후처리조적평균상태시간（50.24±9.72）s명현소우결혈재관주조，차이유통계학의의（t=7.74， P＜0.05）。재관주후제3천，결혈재관주조적상태시간（55.90±11.26）s잉명현장우결혈후처리조（29.22±5.82）s，차이유통계학의의（t=6.65， P＜0.05）。72 h후병이학염색제시：결혈재관주조화결혈후처리조해마CA1구존활신경원분별위가수술조적25.90％화64.60％。결론결혈후처리가이명현감소대서전뇌결혈재관주후신경원사망，감경행위학결손，대대서전뇌결혈재관주유신경보호작용。
Objective To investigate the effect of ischemic postconditioning on global ischemia-reperfusion injury in rats. Methods Thirty male SD rats were randomly assigned to three groups with ten rats each and four blood vessel that blocking the whole cerebral ischemia model was set up. Sham group was just received anesthesia and operation without is-chemia. Ischemia-reperfusion group was received reperfusion at 10 minutes after ischemia. Ischemic postconditioning group was received reperfusion 10 seconds and blocking 10 seconds at 10 minutes after ischemia, thus circling 6 times. Behavior tests were observed at first and third day after reperfusion. Pathology detections were taken at 72 hours after reperfusion. Results Morris Water Maze showed that the latency to platform of ischemia-reperfusion group was(84.76±10.22) seconds which was significantly longer than sham group that was (27.08±7.52) seconds (t=14.84, P<0.05) as well as ischemic postconditioning group that was(50.24±9.72) seconds(t=7.74, P<0.05) at first day after reperfusion. The latency to plat-form of ischemia-reperfusion group was(55.90±11.26) seconds which was significantly longer than ischemic postcondition-ing group that was(29.22±5.82)seconds at third day after reperfusion(t=6.65, P<0.05).The pathologic results showed that the neuron survival rate of ischemia-reperfusion group and ischemic postconditioning group were 25.90% and 64.60%. Conclusion Ischemia postconditioning could reduce the brain neuron death after ischemia reperfusion in rats, attenuate behavioral defects and has neuroprotection on ischemia-reperfusion in rats.
