CAJ | 학술논문

目的：探讨联合靶向Ras和mTOR信号在肝细胞癌(hepatocellular carcinoma，HCC)治疗中的价值。方法：四甲基偶氮唑盐(methylthiazolyl tetrazolium，MTT)检测不同浓度Ras抑制剂(farnesylthiosalicylic acid，FTS)和(或)mTOR抑制剂雷帕霉素对肝癌细胞增殖的影响；流式细胞仪检测细胞凋亡；进一步研究两种药物联合对Balb/c小鼠肝癌移植瘤生长的影响。结果：FTS联合雷帕霉素更能抑制HepG2细胞增殖、抑制小鼠肝癌移植瘤的生长，诱导肝癌细胞凋亡。结论：Ras和mTOR信号在HCC治疗中具有联合靶向价值。
목적：탐토연합파향Ras화mTOR신호재간세포암(hepatocellular carcinoma，HCC)치료중적개치。방법：사갑기우담서염(methylthiazolyl tetrazolium，MTT)검측불동농도Ras억제제(farnesylthiosalicylic acid，FTS)화(혹)mTOR억제제뢰파매소대간암세포증식적영향；류식세포의검측세포조망；진일보연구량충약물연합대Balb/c소서간암이식류생장적영향。결과：FTS연합뢰파매소경능억제HepG2세포증식、억제소서간암이식류적생장，유도간암세포조망。결론：Ras화mTOR신호재HCC치료중구유연합파향개치。
Objective: To investigate the value of combined blockade Ras and mTOR signaling in the therapy of HCC. Methods: Specific Ras and/or mTOR inhibitors were used to inhibit Ras and mTOR relatively. Cell proliferation was assessed by using the MTT assay. Early apoptosis was detected by Annexin-V-FITC/propidium iodide double staining assay. The ef-fects of the two drugs on HCC were also assessed in xenograft models. Results:Ras inhibitor FTS and mTOR inhibitor all in-hibited HepG2 and Huh-7 cell growth, induced cell apoptosis. Conclusion: Con-target Ras and mTOR could markedly in-hibited HCC cell growth in vitro and in vivo.