CAJ | 학술논문

【目的】探讨抑癌基因PTEN及DNA错配修复基因hMSH2在年轻妇女子宫内膜癌组织中的表达及相关性。【方法】选取因子宫病变行子宫全切的45岁及以下妇女子宫内膜组织标本66例，其中子宫内膜腺癌42例、子宫内膜非典型增生6例、正常子宫内膜18例。采用免疫组织化学SP法测定PTEN及hMSH2在子宫内膜组织中的表达。【结果】①在正常子宫内膜、子宫内膜不典型增生及子宫内膜癌中PT EN的表达率逐渐降低，缺失率分别为16．67％、66．67％、85．71％，且差异均有极显著性（ P ＜0．01）；hMSH2的表达率分别为44．44％、33．33％、73．81％，各组之间相比较差异有显著性（ P＜0．05）；②PTEN及hMSH2在子宫内膜癌中的表达与临床分期、组织学分级、肌层浸润深度及有无淋巴转移无关（rs 值＝0．271， P ＞0．05）。③在子宫内膜癌中，hMSH2蛋白表达与PTEN 蛋白表达无显著性相关关系（ P ＜0．05）；hMSH2蛋白与 PTEN蛋白表达呈不一致（χ2＝23．04，P＝0．000）。【结论】同时检测PTEN及hMSH2在年轻妇女子宫内膜癌的表达有助于早期诊断，并能推测预后。
【목적】탐토억암기인PTEN급DNA착배수복기인hMSH2재년경부녀자궁내막암조직중적표체급상관성。【방법】선취인자궁병변행자궁전절적45세급이하부녀자궁내막조직표본66례，기중자궁내막선암42례、자궁내막비전형증생6례、정상자궁내막18례。채용면역조직화학SP법측정PTEN급hMSH2재자궁내막조직중적표체。【결과】①재정상자궁내막、자궁내막불전형증생급자궁내막암중PT EN적표체솔축점강저，결실솔분별위16．67％、66．67％、85．71％，차차이균유겁현저성（ P ＜0．01）；hMSH2적표체솔분별위44．44％、33．33％、73．81％，각조지간상비교차이유현저성（ P＜0．05）；②PTEN급hMSH2재자궁내막암중적표체여림상분기、조직학분급、기층침윤심도급유무림파전이무관（rs 치＝0．271， P ＞0．05）。③재자궁내막암중，hMSH2단백표체여PTEN 단백표체무현저성상관관계（ P ＜0．05）；hMSH2단백여 PTEN단백표체정불일치（χ2＝23．04，P＝0．000）。【결론】동시검측PTEN급hMSH2재년경부녀자궁내막암적표체유조우조기진단，병능추측예후。
[Objective] To explore the expressions of tumor suppressor gene PTEN and DNA mismatch repair gene hMSH2 in young women with endometrial carcinoma and their correlation .[Methods]The endometrial specimens of 66 women(≤45 years old) undergoing total hysterectomy for uterine diseases were collected .Among them ,there were 42 cases of endometrial carcinoma ,6 cases of atypical endometrial hyperplasia and 18 cases of normal endometri-um .The expressions of PTEN and hMSH2 in the tissues of endometrium were detected by immunohistochemical SP method .[Results] The expression rates of PTEN in normal endometrium ,atypical endometrial hyperplasia and endo-metrial carcinoma were decreased gradually ,and the absence rates were 16 .67% ,66 .67% and 85 .71% respectively , and there was significant difference ( P < 0 .01) .The expression rates of hMSH2 were 44 .44% ,33 .33% and 73 .81% ,respectively ,and there was significance difference among groups ( P <0 .05) .The expressions of PTEN and hMSH2 had no correlation with clinical stage ,histological grade ,myometrial invasion depth and lymphatic metas-tasis whether or not(rs=0 .271 ,P>0 .05) .There was no significant correlation between hMSH2 and PTEN in en-dometrial carcinoma( P < 0 .05) .The expression of hMSH2 was discordant with the expression of PTEN (χ2 =23 .04 ,P=0 .000) .[Conclusion] The combined detection of PTEN and hMSH2 is helpful for the early diagnosis and can infer the prognosis .