农业科学与技术（英文版）
農業科學與技術（英文版）
농업과학여기술（영문판）
AGRICULTURAL SCIENCE & TECHNOLOGY
2014年
3期
370-374
,共5页
菊花%叶片%茎段%直接再生
菊花%葉片%莖段%直接再生
국화%협편%경단%직접재생
Dendranthema morifolium%Leaf%Stem%Direct regeneration
以9个菊花品种叶片、茎段为外植体，用6种分化培养基与3种生根培养基直接再生不定芽，研究激素组合及外植体对不同菊花品种直接再生的影响。结果表明，9个品种的茎段与6个品种的叶片都有较高的再生能力，但品种“28”，“5-17”的叶片未分化出不定芽，品种“11”的叶片再生率也不高；多数菊花品种茎段的再生能力高于叶片；再生率为84％～100％，繁殖系数为2．3～9．4，可应用于工厂化快繁与基因工程育种。
以9箇菊花品種葉片、莖段為外植體，用6種分化培養基與3種生根培養基直接再生不定芽，研究激素組閤及外植體對不同菊花品種直接再生的影響。結果錶明，9箇品種的莖段與6箇品種的葉片都有較高的再生能力，但品種“28”，“5-17”的葉片未分化齣不定芽，品種“11”的葉片再生率也不高；多數菊花品種莖段的再生能力高于葉片；再生率為84％～100％，繁殖繫數為2．3～9．4，可應用于工廠化快繁與基因工程育種。
이9개국화품충협편、경단위외식체，용6충분화배양기여3충생근배양기직접재생불정아，연구격소조합급외식체대불동국화품충직접재생적영향。결과표명，9개품충적경단여6개품충적협편도유교고적재생능력，단품충“28”，“5-17”적협편미분화출불정아，품충“11”적협편재생솔야불고；다수국화품충경단적재생능력고우협편；재생솔위84％～100％，번식계수위2．3～9．4，가응용우공엄화쾌번여기인공정육충。
Leaves and stems of 9 chrysanthemum species were taken as explants and inoculated to 6 differential media and root media to research effects of hor-mone combination and explants on chrysanthemum species. The results indicated that stem of 9 chrysanthemum species and leaf of 6 chrysanthemum species al showed higher regeneration capacity, with regeneration rate in 84%-100% and re-generation coefficient at 2.3-9.4. However, none adventitious buds were found differ-entiated from leaves of "28" and "5-17", and leaf regeneration capacity of "11" was lower, either. Most chrysanthemum species proved higher in stem regeneration ca-pacity, instead of leaf. Therefore, chrysanthemum species can be applied for rapid reproduction and genetic engineering.
