医学分子生物学杂志
醫學分子生物學雜誌
의학분자생물학잡지
FOREIGN MEDICAL SCIENCES
2013年
1期
21-25
,共5页
夏曦%蒋学峰%纪腾%徐洪斌
夏晞%蔣學峰%紀騰%徐洪斌
하희%장학봉%기등%서홍빈
转录因子Snail%卵巢癌侵袭转移%上皮间质变(EMT)%e-cadherin%vimentin
轉錄因子Snail%卵巢癌侵襲轉移%上皮間質變(EMT)%e-cadherin%vimentin
전록인자Snail%란소암침습전이%상피간질변(EMT)%e-cadherin%vimentin
目的 研究Snail与卵巢癌侵袭转移间的关系,并探讨其分子机制.方法 分别构建正义全长Snail真核表达载体和小分子干扰RNA,分别转染卵巢癌细胞系A2780、C13.采用Western 印迹方法分析相关蛋白表达,Transwell试验检测细胞侵袭转移能力.结果 ①成功构建Snail正义全长真核表达载体,并合成Snail小分子RNA干扰片段;② 在转染PEGFPC1/Snail的卵巢癌细胞株A2780、C13*中E-cadherin表达明显下调,Snail和Vimentin则表达上调;而在转染Snail/SiRNA的卵巢癌细胞株A2780、C13*中E-cadherin表达明显上调,Snail和Vimentin则表达下调;③ Snail过表达可显著促进卵巢癌细胞株A2780、C13*的侵袭转移潜能;封闭Snail表达水平可一定水平抑制卵巢癌细胞株A2780、C13*的侵袭转移潜能.结论 Snail可通过促进卵巢癌上皮细胞间质化转变(epithelial-mesenchymal transition,EMT)而提高其侵袭转移能力;封闭Snail表达可一定程度逆转卵巢癌上皮细胞间质化转变(EMT)而抑制其侵袭转移能力.
目的 研究Snail與卵巢癌侵襲轉移間的關繫,併探討其分子機製.方法 分彆構建正義全長Snail真覈錶達載體和小分子榦擾RNA,分彆轉染卵巢癌細胞繫A2780、C13.採用Western 印跡方法分析相關蛋白錶達,Transwell試驗檢測細胞侵襲轉移能力.結果 ①成功構建Snail正義全長真覈錶達載體,併閤成Snail小分子RNA榦擾片段;② 在轉染PEGFPC1/Snail的卵巢癌細胞株A2780、C13*中E-cadherin錶達明顯下調,Snail和Vimentin則錶達上調;而在轉染Snail/SiRNA的卵巢癌細胞株A2780、C13*中E-cadherin錶達明顯上調,Snail和Vimentin則錶達下調;③ Snail過錶達可顯著促進卵巢癌細胞株A2780、C13*的侵襲轉移潛能;封閉Snail錶達水平可一定水平抑製卵巢癌細胞株A2780、C13*的侵襲轉移潛能.結論 Snail可通過促進卵巢癌上皮細胞間質化轉變(epithelial-mesenchymal transition,EMT)而提高其侵襲轉移能力;封閉Snail錶達可一定程度逆轉卵巢癌上皮細胞間質化轉變(EMT)而抑製其侵襲轉移能力.
목적 연구Snail여란소암침습전이간적관계,병탐토기분자궤제.방법 분별구건정의전장Snail진핵표체재체화소분자간우RNA,분별전염란소암세포계A2780、C13.채용Western 인적방법분석상관단백표체,Transwell시험검측세포침습전이능력.결과 ①성공구건Snail정의전장진핵표체재체,병합성Snail소분자RNA간우편단;② 재전염PEGFPC1/Snail적란소암세포주A2780、C13*중E-cadherin표체명현하조,Snail화Vimentin칙표체상조;이재전염Snail/SiRNA적란소암세포주A2780、C13*중E-cadherin표체명현상조,Snail화Vimentin칙표체하조;③ Snail과표체가현저촉진란소암세포주A2780、C13*적침습전이잠능;봉폐Snail표체수평가일정수평억제란소암세포주A2780、C13*적침습전이잠능.결론 Snail가통과촉진란소암상피세포간질화전변(epithelial-mesenchymal transition,EMT)이제고기침습전이능력;봉폐Snail표체가일정정도역전란소암상피세포간질화전변(EMT)이억제기침습전이능력.
