CAJ | 학술논문

目的：研究维吾尔族和汉族不同龋敏感儿童牙菌斑中远缘链球菌的检出量，分析远缘链球菌与不同民族儿童乳牙龋齿发生的关系，为维吾尔族和汉族儿童乳牙龋的防治提供依据。方法采用 T-A克隆技术制备远缘链球菌ATCC6715、内参基因16S rRNA的质粒标准品，应用SYBR Green I荧光定量聚合酶链反应对3～5岁维吾尔族和汉族不同龋敏感儿童牙菌斑中远缘链球菌进行绝对定量检测，以龋失补牙数≥5颗为高龋组和龋失补牙数＝0颗为无龋组，使用SPSS17．0软件包对结果进行析因分析。结果维吾尔族儿童高龋组远缘链球菌的检出量为（1．06×105±4．27×103） co pies／μL ，高于无龋组[（2．55×104±2．84×103） co pies／μL ]，差异有统计学意义（ P ＜0．05）；汉族儿童高龋组远缘链球菌的检出量为（8．29×104±1．20×104） co pies／μL ，高于无龋组[（1．54×103±9．74×102） copies／μL]，差异有统计学意义（P ＜0．05），同时维吾尔族儿童远缘链球菌的检出量高于汉族儿童，差异有统计学意义（P ＜0．05）。结论远缘链球菌可能是维吾尔族和汉族儿童乳牙龋高发的危险因素，维吾尔族远缘链球菌高检出量可能与不同生活习俗和饮食习惯有关。
목적：연구유오이족화한족불동우민감인동아균반중원연련구균적검출량，분석원연련구균여불동민족인동유아우치발생적관계，위유오이족화한족인동유아우적방치제공의거。방법채용 T-A극륭기술제비원연련구균ATCC6715、내삼기인16S rRNA적질립표준품，응용SYBR Green I형광정량취합매련반응대3～5세유오이족화한족불동우민감인동아균반중원연련구균진행절대정량검측，이우실보아수≥5과위고우조화우실보아수＝0과위무우조，사용SPSS17．0연건포대결과진행석인분석。결과유오이족인동고우조원연련구균적검출량위（1．06×105±4．27×103） co pies／μL ，고우무우조[（2．55×104±2．84×103） co pies／μL ]，차이유통계학의의（ P ＜0．05）；한족인동고우조원연련구균적검출량위（8．29×104±1．20×104） co pies／μL ，고우무우조[（1．54×103±9．74×102） copies／μL]，차이유통계학의의（P ＜0．05），동시유오이족인동원연련구균적검출량고우한족인동，차이유통계학의의（P ＜0．05）。결론원연련구균가능시유오이족화한족인동유아우고발적위험인소，유오이족원연련구균고검출량가능여불동생활습속화음식습관유관。
Objective To detect the presence of Streptococcus sobrinus in dental plaque of children from Urumqi and correlate it to dental caries ,and to assess their association with the prevalence of deciduous caries .Methods Using the T-A cloning method to construct the standards of S .sobrinus ATCC6715 and Uni for real-time fluorescence quantitative polymerase chain reaction ,sample of 169 children were collect-ed ,70 of which were Uyghur ,between 3 and 5 years′old .Dental plaque samples were collected and bacte-rial DNA extracted ,which subjected to FQ-PCR analysis .The data were statistically analyzed by SPSS 17 .0 software package .Results The quantity of S .sobrinus in plaque of Uyghur children with high dmft was higher than that in caries-free children (S .sobrinus :1 .06 × 105 ± 4 .27 × 103 vs 2 .55 × 104 ± 2 .84 × 103 ,P<0 .05) ,while there was statistically significant difference of HAN people (S .sobrinus :8 .29 × 104 ± 1 .20 × 104 vs 1 .54 × 103 ± 9 .74 × 102 ,P <0 .05) .Conclusion These results indicate that Uyghur and Chinese preschool children harboring S .sobrinus have a significantly high incidence of dental caries .