CAJ | 학술논문

目的：探讨异叶青兰醇提物对肾性高血压大鼠血压的影响及其降压机制。方法利用肾动脉狭窄法建立两肾一夹高血压大鼠模型；将成模的高血压大鼠随机分为假手术组、模型组、异叶青兰高剂量组（D HBE-H）、异叶青兰低剂量组（DHBE-L）、卡托普利组；灌胃给药5 w ，每周无创尾套法测量大鼠尾动脉血压；5 w 后用颈总动脉插管测血流动力学指标，采用硝酸还原酶法测定心肌组织中一氧化氮（N O ）水平，化学比色法测定心肌组织中一氧化氮合成酶（NOS）水平，酶联免疫吸附法（ELISA）测定心肌组织中内皮素（ET）和血管紧张素Ⅱ（AngII）水平。结果与模型组相比，异叶青兰醇提物能使肾性高血压大鼠尾动脉收缩压明显降低；颈动脉收缩压（SBP）、舒张压（DBP）、左室收缩压（LVSP）、降低左室舒张末期压（LVEDP）均显著降低，室内压对数值最大变化速率（± dp／dtmax）／LVSP和收缩指数（CI）升高，NO水平升高，ET水平降低；AngII水平降低。结论异叶青兰醇提物能降低肾性高血压大鼠血压，增强心脏舒缩功能。
목적：탐토이협청란순제물대신성고혈압대서혈압적영향급기강압궤제。방법이용신동맥협착법건립량신일협고혈압대서모형；장성모적고혈압대서수궤분위가수술조、모형조、이협청란고제량조（D HBE-H）、이협청란저제량조（DHBE-L）、잡탁보리조；관위급약5 w ，매주무창미투법측량대서미동맥혈압；5 w 후용경총동맥삽관측혈류동역학지표，채용초산환원매법측정심기조직중일양화담（N O ）수평，화학비색법측정심기조직중일양화담합성매（NOS）수평，매련면역흡부법（ELISA）측정심기조직중내피소（ET）화혈관긴장소Ⅱ（AngII）수평。결과여모형조상비，이협청란순제물능사신성고혈압대서미동맥수축압명현강저；경동맥수축압（SBP）、서장압（DBP）、좌실수축압（LVSP）、강저좌실서장말기압（LVEDP）균현저강저，실내압대수치최대변화속솔（± dp／dtmax）／LVSP화수축지수（CI）승고，NO수평승고，ET수평강저；AngII수평강저。결론이협청란순제물능강저신성고혈압대서혈압，증강심장서축공능。
Objective To observe the effects of Dracocephalum heterophyllum Benth (DHBE) on blood pressure in renovascular hypertensive rats (RHR) and explore its mechanisms .Methods Two-kidney-one-clip RHRs were obtained by narrowing left kidney arteries .6 groups were divided as control ,model ,DH-BE high dose group (DHBE-H) ,DHBE low dose group (DHBE-L) and captopril groups .The drugs were given by intragastric administration for 5 weeks .Systolic blood pressure (SBP) were measured by tail cuff approach every week .After the fifth week ,we utilized left ventricular incubation to measure hemodynamic parameters .The levels of nitric oxide (NO) ,nitric oxide synthase (NOS) in heart were respectively meas-ured by nitrate reductase method and chemical colorimetry .The levels of endothelin (ET ) and AngⅡ in heart were measured by enzyme linked immunosorbent assay (ELISA) approach .Results Compared with model group ,DHBE significantly reduced RHR blood pressure ,systolic blood pressure (SBP) ,diastolic blood pressure (DBP) ,left ventricular systolic pressure (LVSP) ,left ventricular end-diastolic pressure (LVEDP) and increased (± dp/dtmax)/LVSP and CI ,increased the concentration of NO while decrease the content of ET ,and level of Ang Ⅱ .Conclusion DHBE could reduce RHR blood pressure ,and im-prove cardiac systolic and diastolic functions .