CAJ | 학술논문

目的：探讨黑木耳提取物对感染性休克大鼠肝、肠组织丙二醛（MDA）及谷胱甘肽过氧化物酶（GSH-Px）的影响。方法将50只健康Wister大鼠随机分为对照组（假手术组）、模型组（感染性休克组）、阳性对照组（思密达组）、黑木耳粗提物组、黑木耳多糖组5组，每组10只。各组大鼠连续灌胃3d，每d3次，分别灌注生理盐水、生理盐水、思密达、黑木耳粗提物、黑木耳多糖，灌注量为1．5 m L／100 g体重。在乙醚麻醉下行盲肠结扎穿刺术，6 h后取大鼠腹主动脉血液及时检测血气，取肝、肠及肺组织光镜下观察形态学变化，并检测肝、肠组织MDA及GSH-Px的含量。结果与对照组比较，模型组大鼠肝、肠内 MDA 浓度升高，黑木耳粗提物组大鼠肝脏内MDA浓度升高，差异有统计学意义（P ＜0．05）；与模型组比较，思密达组和黑木耳多糖组大鼠肝、肠组织内MDA浓度降低，黑木耳粗提物组大鼠小肠内MDA浓度降低，差异有统计学意义（P ＜0．05）。与对照组比较，模型组和黑木耳粗提物组大鼠小肠内GS H-Px水平下降，差异有统计学意义（ P ＜0．05）；与模型组比较，思密达组和黑木耳多糖组大鼠小肠内GS H-Px水平升高，差异有统计学意义（ P ＜0．05）；与对照组比较，其余4组大鼠肝脏内GS H-Px浓度均降低，差异有统计学意义（ P ＜0．05）；与模型组比较，黑木耳粗提物组和黑木耳多糖组大鼠肝脏内GS H-Px浓度升高，差异有统计学意义（P ＜0．05）；与思密达组比较，黑木耳多糖组GSH-Px浓度升高，差异有统计学意义（P ＜0．05）。光镜观察：对照组肝脏及小肠组织正常，模型组肝细胞呈片状水肿，小肠黏膜上皮细胞破坏，肺间质水肿，其余3组组织上述表现则较轻，基本正常。结论黑木耳多糖能有效控制感染性休克时小肠和肝脏内有害物质MDA上升的水平，减缓GSH-Px下降的水平，且其对肝脏内GSH-Px的影响优于思密达。目的：探討黑木耳提取物對感染性休剋大鼠肝、腸組織丙二醛（MDA）及穀胱甘肽過氧化物酶（GSH-Px）的影響。方法將50隻健康Wister大鼠隨機分為對照組（假手術組）、模型組（感染性休剋組）、暘性對照組（思密達組）、黑木耳粗提物組、黑木耳多糖組5組，每組10隻。各組大鼠連續灌胃3d，每d3次，分彆灌註生理鹽水、生理鹽水、思密達、黑木耳粗提物、黑木耳多糖，灌註量為1．5 m L／100 g體重。在乙醚痳醉下行盲腸結扎穿刺術，6 h後取大鼠腹主動脈血液及時檢測血氣，取肝、腸及肺組織光鏡下觀察形態學變化，併檢測肝、腸組織MDA及GSH-Px的含量。結果與對照組比較，模型組大鼠肝、腸內 MDA 濃度升高，黑木耳粗提物組大鼠肝髒內MDA濃度升高，差異有統計學意義（P ＜0．05）；與模型組比較，思密達組和黑木耳多糖組大鼠肝、腸組織內MDA濃度降低，黑木耳粗提物組大鼠小腸內MDA濃度降低，差異有統計學意義（P ＜0．05）。與對照組比較，模型組和黑木耳粗提物組大鼠小腸內GS H-Px水平下降，差異有統計學意義（ P ＜0．05）；與模型組比較，思密達組和黑木耳多糖組大鼠小腸內GS H-Px水平升高，差異有統計學意義（ P ＜0．05）；與對照組比較，其餘4組大鼠肝髒內GS H-Px濃度均降低，差異有統計學意義（ P ＜0．05）；與模型組比較，黑木耳粗提物組和黑木耳多糖組大鼠肝髒內GS H-Px濃度升高，差異有統計學意義（P ＜0．05）；與思密達組比較，黑木耳多糖組GSH-Px濃度升高，差異有統計學意義（P ＜0．05）。光鏡觀察：對照組肝髒及小腸組織正常，模型組肝細胞呈片狀水腫，小腸黏膜上皮細胞破壞，肺間質水腫，其餘3組組織上述錶現則較輕，基本正常。結論黑木耳多糖能有效控製感染性休剋時小腸和肝髒內有害物質MDA上升的水平，減緩GSH-Px下降的水平，且其對肝髒內GSH-Px的影響優于思密達。
목적：탐토흑목이제취물대감염성휴극대서간、장조직병이철（MDA）급곡광감태과양화물매（GSH-Px）적영향。방법장50지건강Wister대서수궤분위대조조（가수술조）、모형조（감염성휴극조）、양성대조조（사밀체조）、흑목이조제물조、흑목이다당조5조，매조10지。각조대서련속관위3d，매d3차，분별관주생리염수、생리염수、사밀체、흑목이조제물、흑목이다당，관주량위1．5 m L／100 g체중。재을미마취하행맹장결찰천자술，6 h후취대서복주동맥혈액급시검측혈기，취간、장급폐조직광경하관찰형태학변화，병검측간、장조직MDA급GSH-Px적함량。결과여대조조비교，모형조대서간、장내 MDA 농도승고，흑목이조제물조대서간장내MDA농도승고，차이유통계학의의（P ＜0．05）；여모형조비교，사밀체조화흑목이다당조대서간、장조직내MDA농도강저，흑목이조제물조대서소장내MDA농도강저，차이유통계학의의（P ＜0．05）。여대조조비교，모형조화흑목이조제물조대서소장내GS H-Px수평하강，차이유통계학의의（ P ＜0．05）；여모형조비교，사밀체조화흑목이다당조대서소장내GS H-Px수평승고，차이유통계학의의（ P ＜0．05）；여대조조비교，기여4조대서간장내GS H-Px농도균강저，차이유통계학의의（ P ＜0．05）；여모형조비교，흑목이조제물조화흑목이다당조대서간장내GS H-Px농도승고，차이유통계학의의（P ＜0．05）；여사밀체조비교，흑목이다당조GSH-Px농도승고，차이유통계학의의（P ＜0．05）。광경관찰：대조조간장급소장조직정상，모형조간세포정편상수종，소장점막상피세포파배，폐간질수종，기여3조조직상술표현칙교경，기본정상。결론흑목이다당능유효공제감염성휴극시소장화간장내유해물질MDA상승적수평，감완GSH-Px하강적수평，차기대간장내GSH-Px적영향우우사밀체。
Objective To study the effects of extract from black fungus on liver and small intestine′s malon-dialdehyde (MDA) ,glutathione peroxidase (GSH-Px) in the septic shock rat ,as well as the protection of the liver and small intestine′s function during the failture caused by septic shock .Methods Fifty healthy Wistar rats were randomly divided into 5 groups:the blank control (the sham-operation) group ,the tes-ting (the septic) group ,the smectite(the montmorillonite power) group ,the black fungus crude extract group and the black fungus polysaccharids group .Respectively undertook saline ,saline ,smectite ,black fungus crude extract ,black fungus polysaccharids to each group on three consecutive days .And the perfu-sion was 1 .5 mL/100 g ,three times a day .Then it was filled again 180 min before establishing the mod-els .Established the models by performing ligation and perforation on cecum under the ether .After 6h , draw off blood of the abdominal aortic to analyze blood-gas ,and observed the form on liver ,small intestine and lung ,as well as measured the content of MDA ,GXH-Px in liver and small intestine .Results The concentration of MDA in liver ,small intestine of the testing group and the concentration in liver of the black fungus crude extract group were higher than it of control group ,the differences were statistical sig-nificant (P <0 .05);MDA in liver ,small intestine of the smectite group ,the black fungus polysaccharids group and in small intestine of black fungus crude extract group were lower than it of testing group ,the differences were statistical significant (P <0 .05) .The concentration of GSH-Px in the testing group ,the black fungus crude extract group were lower than it of control group ,the differences were statistical sig-nificant (P <0 .05);The concentration of the smectite group ,the black fungus polysaccharids group were higher than it of testing group ,the differences were statistical significant (P <0 .05);The concentration of GSH-Px in the last four groups were lower than it of control group ,the differences were statistical sig-nificant (P <0 .05) .The concentration of GSH-Px in the black fungus crude extract group and the black fungus polysaccharids group were higher than it of testing group ,the differences were statistical significant (P <0 .05) .The concentration of GSH-Px in the black fungus polysaccharids group were higher than it of smectite group ,the differences were statistical significant (P <0 .05) .Observed under the light micro-scope:The liver ,small intestine of the control group were normal .In the testing group ,the cell of liver were edema ,the epithelial cells of mucosa were damaged .But the above-mentioned performances in the other three groups were less ,practically approaching normal .Conclusion The black fungus polysacchar-ids can control the harmful substance-MDA to rise and slow down the decreasing of the GSH-PX in the septic shock ,as well as it was better than smectite on GSH-Px of liver .