CAJ | 학술논문

目的：探讨嘌呤受体P2X7在体外培养大鼠视网膜毛细血管周细胞上的功能意义。 　　方法：利用显微镜下观察结合TUNEL 计数法分别测定P2 X7激动剂BzATP和拮抗剂OxATP对体外培养的正常视网膜毛细血管周细胞以及糖尿病大鼠视网膜毛细血管周细胞存活率的影响。 　　结果：P2 X 7受体激动后可介导视网膜毛细血管周细胞死亡，糖尿病大鼠视网膜毛细血管周细胞较正常视网膜毛细血管周细胞，在同等激动剂浓度下或在同样的时间点，能引起更多的细胞凋亡。而拮抗剂OxATP 可以明显减轻BzATP对视网膜毛细血管周细胞的毒性作用，提高其存活率。 　　结论：P2 X 7受体活性对视网膜毛细血管周细胞凋亡有一定调控作用。
목적：탐토표령수체P2X7재체외배양대서시망막모세혈관주세포상적공능의의。 　　방법：이용현미경하관찰결합TUNEL 계수법분별측정P2 X7격동제BzATP화길항제OxATP대체외배양적정상시망막모세혈관주세포이급당뇨병대서시망막모세혈관주세포존활솔적영향。 　　결과：P2 X 7수체격동후가개도시망막모세혈관주세포사망，당뇨병대서시망막모세혈관주세포교정상시망막모세혈관주세포，재동등격동제농도하혹재동양적시간점，능인기경다적세포조망。이길항제OxATP 가이명현감경BzATP대시망막모세혈관주세포적독성작용，제고기존활솔。 　　결론：P2 X 7수체활성대시망막모세혈관주세포조망유일정조공작용。
AIM: To discuss the functional significance of purine receptor P2X7 in retinal capillary pericytes rats. METHODS:The TUNEL test was applied to observe the survival rate of normal retinal capillary pericytes and diabetic retinal capillary pericytes which were affected by P2X7 agonist BzATP and antagonist OxATP in microscopy. RESULTS: Activation of P2X7 receptors caused occurence of apoptosis in retinal capillary pericytes. Compared with normal retinal capillary pericytes, when they were at the same concentration or time, the diabetic retinal capillary pericytes caused much more apoptosis than normal retinal capillary pericytes. The antagonist OxATP could significantly reduce the toxic effects activated by BzATP, and then increased the livability of retinal capillary pericytes. CONCLUSION:The activity of P2X7 receptor plays a regulatory role on RPC apoptosis.