国际眼科杂志
國際眼科雜誌
국제안과잡지
INTERNATIONAL JOURNAL OF OPHTHALMOLOGY
2014年
4期
588-592
,共5页
青光眼%神经保护%视网膜%孕酮%凋亡%Caspase-3
青光眼%神經保護%視網膜%孕酮%凋亡%Caspase-3
청광안%신경보호%시망막%잉동%조망%Caspase-3
glaucoma%nerve protection%retina%progesterone%apoptosis%Caspase-3
目的：通过建立大鼠急性高眼压模型，应用孕酮干预，观察孕酮是否对视网膜神经细胞具有保护作用。<br> 方法：Wistar大鼠140只，随机分为正常对照组（ A组）20只、高眼压对照组（B 组）60只、高眼压孕酮干预组（C组）60只。应用生理盐水前房灌注的方法建立大鼠急性高眼压模型，C 组在高眼压后即刻予腹腔注射孕酮4.0mg／kg，以后于6，24h以及隔日行皮下注射，B组于相同时间注射等量的生理盐水。于高眼压后1，3，7d分别获取各组大鼠的视网膜组织。 HE染色观察视网膜病理学的变化，用免疫组化染色、Western blot 检测视网膜细胞凋亡基因Caspase-3蛋白的表达情况， TUNEL法检测细胞的凋亡程度，并对各组数据进行统计学分析。<br> 结果：B组和C组的大鼠视网膜均发生水肿，C组水肿程度明显低于B组。免疫组化染色及Western blot 相对定量检测，C组Caspase-3表达程度低于B组。 Caspase-3蛋白主要表达于视网膜神经节细胞层和内核层，于高眼压后第1d 强度最大，以后随时间的推移逐渐减少。TUNEL检测细胞凋亡，B组和C组均出现阳性细胞，主要位于视网膜的节细胞层和内核层，C组细胞凋亡数量明显低于B组。<br> 结论：孕酮对大鼠视网膜急性高眼压损伤有保护作用。
目的：通過建立大鼠急性高眼壓模型，應用孕酮榦預，觀察孕酮是否對視網膜神經細胞具有保護作用。<br> 方法：Wistar大鼠140隻，隨機分為正常對照組（ A組）20隻、高眼壓對照組（B 組）60隻、高眼壓孕酮榦預組（C組）60隻。應用生理鹽水前房灌註的方法建立大鼠急性高眼壓模型，C 組在高眼壓後即刻予腹腔註射孕酮4.0mg／kg，以後于6，24h以及隔日行皮下註射，B組于相同時間註射等量的生理鹽水。于高眼壓後1，3，7d分彆穫取各組大鼠的視網膜組織。 HE染色觀察視網膜病理學的變化，用免疫組化染色、Western blot 檢測視網膜細胞凋亡基因Caspase-3蛋白的錶達情況， TUNEL法檢測細胞的凋亡程度，併對各組數據進行統計學分析。<br> 結果：B組和C組的大鼠視網膜均髮生水腫，C組水腫程度明顯低于B組。免疫組化染色及Western blot 相對定量檢測，C組Caspase-3錶達程度低于B組。 Caspase-3蛋白主要錶達于視網膜神經節細胞層和內覈層，于高眼壓後第1d 彊度最大，以後隨時間的推移逐漸減少。TUNEL檢測細胞凋亡，B組和C組均齣現暘性細胞，主要位于視網膜的節細胞層和內覈層，C組細胞凋亡數量明顯低于B組。<br> 結論：孕酮對大鼠視網膜急性高眼壓損傷有保護作用。
목적：통과건립대서급성고안압모형，응용잉동간예，관찰잉동시부대시망막신경세포구유보호작용。<br> 방법：Wistar대서140지，수궤분위정상대조조（ A조）20지、고안압대조조（B 조）60지、고안압잉동간예조（C조）60지。응용생리염수전방관주적방법건립대서급성고안압모형，C 조재고안압후즉각여복강주사잉동4.0mg／kg，이후우6，24h이급격일행피하주사，B조우상동시간주사등량적생리염수。우고안압후1，3，7d분별획취각조대서적시망막조직。 HE염색관찰시망막병이학적변화，용면역조화염색、Western blot 검측시망막세포조망기인Caspase-3단백적표체정황， TUNEL법검측세포적조망정도，병대각조수거진행통계학분석。<br> 결과：B조화C조적대서시망막균발생수종，C조수종정도명현저우B조。면역조화염색급Western blot 상대정량검측，C조Caspase-3표체정도저우B조。 Caspase-3단백주요표체우시망막신경절세포층화내핵층，우고안압후제1d 강도최대，이후수시간적추이축점감소。TUNEL검측세포조망，B조화C조균출현양성세포，주요위우시망막적절세포층화내핵층，C조세포조망수량명현저우B조。<br> 결론：잉동대대서시망막급성고안압손상유보호작용。
AIM:To observe whether progesterone has a protective effect on retinal neural cells by establishing the a cute high intraocular pressure model and intervening with progesterone. <br> METHODS: Totally 140 Wistar rats were randomly divided into normal control group ( group A) 20, high intraocular pressure control group ( group B) 60 and high intraocular pressure progesterone treatment group ( group C ) 60. The acute high intraocular pressure models were made by saline anterior chamber perfusion. Group C were treated with intraperitoneal injection of progesterone 4.0mg/Kg immediately after intraocular pressure restored, and after 6, 24h and every other day were treated with subcutaneous injection.Group B were injected with equivalent saline at the same time.At the 1, 3, 7d after ocular hypertension, the retinas of rats in each group were obtained respectively. Retinas pathological changes were observed in samples hemetoxylin and eosin staining.The protein expression of Caspase-3 was measured with immunohistochemistry and Western blot.Apoptosis in retinal nerve cells were tested with TUNEL technique. Statistical analysis was performed in each group. <br> RESULTS:The rats retina of group B and group C were edema, but the degree in group C was lower than that in group B. In relative quantitative detection of immunohistochemistry and Western blot,expression of C aspase -3 was lower than group B. Substantial expression of Caspase-3 protein lay in cell nucleus and cytoplasm.The level of Caspase-3 in group B was higher than in group C.The positive cells were mainly in inner nucleus layer and ganglion cell layer.The level on the first day after high intraocular pressure was the highest, then gradually decreased along with the passage of time. Apoptosis tested with TUNEL technique, positive cells could be observed in group B and group C, which in inner nucleus layer and ganglion cell layer.But the number in group B was significantly more than group C. <br> CONCLUSION: Progesterone has nerve protection functions on retinal acute high intraocular pressure injury in rats.
