检验医学与临床
檢驗醫學與臨床
검험의학여림상
JOURNAL OF LABORATORY MEDICINE AND CLINICAL SCIENCES
2014年
7期
906-907
,共2页
胃癌%纤溶酶原激活物抑制剂-1%基因频率%多态性
胃癌%纖溶酶原激活物抑製劑-1%基因頻率%多態性
위암%섬용매원격활물억제제-1%기인빈솔%다태성
gastric cancer%plasminogen activator inhibitor-1%gene frequency%polymorphism
目的：探讨纤溶酶原激活物抑制剂-1（PAI-1）基因启动子区的一个鸟嘌呤插入／缺失多态性（4G／5G）与胃癌侵袭和转移的关系。方法运用聚合酶链反应（PCR）和琼脂糖电泳对28例胃癌患者癌变胃组织与癌旁正常胃组织进行PAI-l基因4G／5G多态性检测和分析，并将两者进行比较。结果胃癌患者PAI-13种基因型在癌变胃组织和癌旁正常胃组织的表达差异无统计学意义（P＞0．05），且在胃癌各期表达差异亦无统计学意义（P＞0．05）。有淋巴结转移组的胃癌患者4G／4G基因型频率为52．6％，4G／5G基因型频率为47．4％，5G／5G基因型频率为0％；无淋巴结转移组分别为22．2％、55．6％和22．2％，两组基因型频率比较差异有统计学意义（ P＜0．05），其中4G／4G基因型相对于其他基因型差异有统计学意义（P＜0．05）。结论 PAI-1基因多态性与胃癌的侵袭和TNM分期无关，而与胃癌淋巴结转移相关，4G／4G型可能是胃癌淋巴结转移的风险因素。
目的：探討纖溶酶原激活物抑製劑-1（PAI-1）基因啟動子區的一箇鳥嘌呤插入／缺失多態性（4G／5G）與胃癌侵襲和轉移的關繫。方法運用聚閤酶鏈反應（PCR）和瓊脂糖電泳對28例胃癌患者癌變胃組織與癌徬正常胃組織進行PAI-l基因4G／5G多態性檢測和分析，併將兩者進行比較。結果胃癌患者PAI-13種基因型在癌變胃組織和癌徬正常胃組織的錶達差異無統計學意義（P＞0．05），且在胃癌各期錶達差異亦無統計學意義（P＞0．05）。有淋巴結轉移組的胃癌患者4G／4G基因型頻率為52．6％，4G／5G基因型頻率為47．4％，5G／5G基因型頻率為0％；無淋巴結轉移組分彆為22．2％、55．6％和22．2％，兩組基因型頻率比較差異有統計學意義（ P＜0．05），其中4G／4G基因型相對于其他基因型差異有統計學意義（P＜0．05）。結論 PAI-1基因多態性與胃癌的侵襲和TNM分期無關，而與胃癌淋巴結轉移相關，4G／4G型可能是胃癌淋巴結轉移的風險因素。
목적：탐토섬용매원격활물억제제-1（PAI-1）기인계동자구적일개조표령삽입／결실다태성（4G／5G）여위암침습화전이적관계。방법운용취합매련반응（PCR）화경지당전영대28례위암환자암변위조직여암방정상위조직진행PAI-l기인4G／5G다태성검측화분석，병장량자진행비교。결과위암환자PAI-13충기인형재암변위조직화암방정상위조직적표체차이무통계학의의（P＞0．05），차재위암각기표체차이역무통계학의의（P＞0．05）。유림파결전이조적위암환자4G／4G기인형빈솔위52．6％，4G／5G기인형빈솔위47．4％，5G／5G기인형빈솔위0％；무림파결전이조분별위22．2％、55．6％화22．2％，량조기인형빈솔비교차이유통계학의의（ P＜0．05），기중4G／4G기인형상대우기타기인형차이유통계학의의（P＜0．05）。결론 PAI-1기인다태성여위암적침습화TNM분기무관，이여위암림파결전이상관，4G／4G형가능시위암림파결전이적풍험인소。
Objective To investigate the relationship between a guanine sub region of the insertion/deletion polymorphism (4G/5G) in the plasminogen activator inhibitor-1 (PAI-1) gene promoter and the invasion and metas-tasis of gastric cancer .Methods Polymerase chain reaction and agarose gel electrophoresis were performed to analyze the PAI-l gene 4G/5G polymorphism in cancer tissues and adjacent normal gastric tissues in 28 cases of gastric canc-er .Results Three genotypes of PAI-1 in gastric cancer tissues and adjacent normal gastric tissue were without signif-icant differences (P>0 .05) in patients with gastric cancer ,and the genotypes of PAI-1 were also without differences between patients at different TNM stages (P>0 .05) .The genotype frequency of 4G/4G in gastric cancer patients with lymph node metastasis was 52 .6% ,of 4G/5G was 47 .4% and of 5G/5G was 0 .0% ,which in patients without lymph node metastasis were 22 .2% ,55 .6% and 22 .2% .Genotype frequency of these two groups were with statisti-cal difference (P<0 .05) ,and genotype frequency of 4G/4G was significantly different with the other genotypes (P<0 .05) .Conclusion Polymorphism of PAI-1 gene might be not related to the invasion of gastric cancer and TNM sta-ges ,but closely related to lymph node metastasis of gastric carcinoma .Genotype of 4G/4G could be the risk factor in lymph node metastasis of gastric cancer .
