CAJ | 학술논문

目的：研究建立人食管癌裸鼠皮下移植及原位移植模型的方法。方法从新鲜的人食管癌标本上切除肿瘤组织，移植于裸鼠皮下，建立原代移植瘤并连续传代3次后，取肿瘤组织分别移植于裸鼠皮下及食管黏膜下，分别观察移植瘤成瘤率、大体及组织形态、肿瘤的侵袭和转移情况。结果皮下移植模型成瘤率60％，为低分化鳞癌，未见淋巴结及远处器官转移；原位移植模型成瘤率80％，与患者原发肿瘤组织形态一致，可见广泛的淋巴结转移及肝肺转移。结论初步建立了有效的人食管癌裸鼠皮下移植及原位移植模型，为食管癌的研究提供了重要工具。
목적：연구건립인식관암라서피하이식급원위이식모형적방법。방법종신선적인식관암표본상절제종류조직，이식우라서피하，건립원대이식류병련속전대3차후，취종류조직분별이식우라서피하급식관점막하，분별관찰이식류성류솔、대체급조직형태、종류적침습화전이정황。결과피하이식모형성류솔60％，위저분화린암，미견림파결급원처기관전이；원위이식모형성류솔80％，여환자원발종류조직형태일치，가견엄범적림파결전이급간폐전이。결론초보건립료유효적인식관암라서피하이식급원위이식모형，위식관암적연구제공료중요공구。
Objective To establish a subcutaneous and orthotopic transplatation tumor model of human esophageal cancer in nude mice .Methods Fresh tissues of human esophageal cancer were collected and transplanted into subcutaneous places of nude mice .The successfully transplanted primary generation tumor was passed down three times .Then tumor were implanted into the subcutaneous and esophageal submucous tissues of nude mice .The formation rate ,morphology ,invasion and metastasis of tumor were observed .Results The tumor formation rate of subcutaneous transplatation model was 60% ,it was low grade squamous cell carcinoma without lymph node and distant organ metastasis .The tumor formation rate of orthotopic transplatation model was 80% , the morphological characteristics were consistent with human original tumor .Lymph node and organ metastasis could be found ex-tensive .Conclusion We preliminary established effective subcutaneous and orthotopic transplatation tumor model of human esopha-geal cancer in nude mice .It might be an available model for further research of esophageal cancer .