CAJ | 학술논문

目的：探讨livin在内毒素急性肾损伤大鼠肾组织中的表达情况及其在急性肾损伤细胞凋亡中的作用。方法建立内毒素急性肾损伤大鼠模型，应用苏木精-伊红染色并检测血肌酐、尿素氮水平观察肾损伤情况；采用免疫组织化学，观察不同时间点上，内毒素急性肾损伤大鼠肾组织中livin和caspase-3的表达情况，分析其变化与肾损伤的关系。结果与对照组比较，内毒素大鼠肾组织病理形态学损伤明显，血肌酐及尿素氮水平显著升高（P＜0．01），存在急性肾损伤表现。免疫组织化学检测提示，livin和caspase-3在内毒素大鼠肾组织中均有阳性表达（P＜0．01），且在livin出现较高水平表达后，随时间推移，caspase-3表达增强趋势变缓。结论 livin参与了内毒素急性肾损伤的发病机制，并可能通过抑制caspase-3等重要凋亡效应蛋白，发挥减轻肾损伤、保护肾功能的作用。
목적：탐토livin재내독소급성신손상대서신조직중적표체정황급기재급성신손상세포조망중적작용。방법건립내독소급성신손상대서모형，응용소목정-이홍염색병검측혈기항、뇨소담수평관찰신손상정황；채용면역조직화학，관찰불동시간점상，내독소급성신손상대서신조직중livin화caspase-3적표체정황，분석기변화여신손상적관계。결과여대조조비교，내독소대서신조직병리형태학손상명현，혈기항급뇨소담수평현저승고（P＜0．01），존재급성신손상표현。면역조직화학검측제시，livin화caspase-3재내독소대서신조직중균유양성표체（P＜0．01），차재livin출현교고수평표체후，수시간추이，caspase-3표체증강추세변완。결론 livin삼여료내독소급성신손상적발병궤제，병가능통과억제caspase-3등중요조망효응단백，발휘감경신손상、보호신공능적작용。
Objective To investigate the expression of livin of kidney tissues in rats with acute kidney injury (AKI) induced by endotoxin ,and the role of livin in the cell apoptosis of AKI .Methods The rat models with AKI were induced by endotoxin .The de-gree of kidney injury was observed by hematoxylin and eosin stain ,measuring the levels of the serum creatinine and urea nitrogen . The expression of livin and caspase-3 in kidney tissue at different time points was analyzed by immunohistochemistry assay ,and the relationship between the expression of livin and caspase-3 and kidney injury was analyzed .Results Compared to the control group , the rats injected endotoxin had the performance of AKI ,with obviously pathomorphological damage in the kidney tissues ,signifi-cantly increasing in the serum creatinine and urea nitrogen (P<0 .01) .The livin and caspase-3 of kidney tissues in rats with AKI caused by endotoxin were positive expression (P<0 .01) .With the lapse of time ,the trend of increasing of caspase-3 showed gradu-ally slow after the highest expression of livin .Conclusion The livin involved in the pathogenesis of endotoxin-induced AKI ,it might relieve the kidney injury and protect renal function by inhibiting casepase-3 important apoptotic effector protein .