CAJ | 학술논문

目的：观察全反式维甲酸（ATRA）对人肺腺癌细胞A549增殖的影响，并对其机制进行初步的探讨。方法以肺腺癌A549细胞株为研究对象，用噻唑蓝（MTT）法检测试验组和对照组细胞，计算细胞的生长抑制率；用透射电镜观察细胞细微结构变化情况；用酶联免疫吸附试验测定细胞上清液中环氧化酶-2（COX-2）的水平变化。结果不同试验浓度的ATRA处理肺腺癌A549细胞，均能对A549细胞产生不同程度的抑制作用，且经过72h后，各试验浓度组均可见COX-2在细胞质中的表达明显下降，并与浓度呈正相关，差异有统计学意义（P＜0．05）；同时，检测（1×10-5mol／L、1×10-4mol／L）试验组中肿瘤坏死因子相关凋亡配体（TRAIL）表达水平，发现经ATRA处理后，A549细胞上清液中TRAIL表达升高。结论ATRA对肺癌细胞A549具有显著抑瘤效应，此效应呈浓度与时间依赖性；在肺癌组织中ATRA可能通过诱导凋亡发挥抑瘤效应，可为肺癌治疗提供理论依据。
목적：관찰전반식유갑산（ATRA）대인폐선암세포A549증식적영향，병대기궤제진행초보적탐토。방법이폐선암A549세포주위연구대상，용새서람（MTT）법검측시험조화대조조세포，계산세포적생장억제솔；용투사전경관찰세포세미결구변화정황；용매련면역흡부시험측정세포상청액중배양화매-2（COX-2）적수평변화。결과불동시험농도적ATRA처리폐선암A549세포，균능대A549세포산생불동정도적억제작용，차경과72h후，각시험농도조균가견COX-2재세포질중적표체명현하강，병여농도정정상관，차이유통계학의의（P＜0．05）；동시，검측（1×10-5mol／L、1×10-4mol／L）시험조중종류배사인자상관조망배체（TRAIL）표체수평，발현경ATRA처리후，A549세포상청액중TRAIL표체승고。결론ATRA대폐암세포A549구유현저억류효응，차효응정농도여시간의뢰성；재폐암조직중ATRA가능통과유도조망발휘억류효응，가위폐암치료제공이론의거。
Objective To explore the influence of all-trans retinoic acid(ATRA) on in-vitro proliferation of human lung adeno-carcinoma cell line A549 and to preliminarily study its mechanism .Methods The human lung adenocarcinoma cell line A549 was taken as the experimental group .The cells in the experimental group and the control group were detected by using 3-(4 ,5-dimethyl-thiazol-2-yl)-2 ,5-diphenyltetrazolium bromide(MTT) assay for calculating the cell growth inhibition rate ;the microstructure of the cultured cells was observed by the transmission electron microscope technique ;the expression of cyclooxygenase-2(COX-2) in the cell supernate was detected by the enzyme linked immunosorbent assay .Results The different concentrations of ATRA for treating A549 cells could produce the inhibiting effect on A 549 cells to some different degrees ,furthermore ,after 72 h ,the expression of COX-2 in cytoplasma among the various concentrations groups was significantly decreased ,which was positively correlated with the concentration ,the difference showing statistical significance (P< 0 .05);at the same time ,by detecting TRAIL expression level in the experimental group(1 × 10-5 mol/L ,1 × 10-4 mol/) ,the expression of TRAIL in the A549 cell supernate after ATRA treatment was increased .Conclusion ATRA has the significant anti-tumor effect on the lung adenocarcinoma cell line A549 with the concen-tration-time dependence;ATRA in lung cancer tissue may play its anti-tumor effect by inducing apoptosis ,which can provide the theoretical basis for the treatment of lung cancer .