CAJ | 학술논문

目的：比较介入法建立比格犬与小型猪两组动物急性心肌梗死模型的成功率及风险，为选择更合适的动物建模提供理论基础。方法比格犬和五指山小型猪各6头，全身麻醉下行右侧股动脉切开穿刺术，左冠状动脉造影后选择冠状动脉球囊送至前降支中段行缺血预适应，并持续封堵180 min后撤出球囊，术前、术后均连续观察心电图变化，术后7 d处死动物取出心脏行病理学检查。结果比格犬组均存活，小型猪组死亡1例（1／6）；比格犬组发生心肌梗死1例（1／6），小型猪组5例（5／6）；比格犬组未发生恶性心律失常，小型猪组6例均发生恶性心律失常（6／6）；两组造模所需时间比较[（4．61±0．47）h vs．（4．82±0．55）h]，差异无统计学意义（P＞0．05）。结论小型猪在介入法建立心肌梗死模型中虽然风险高于比格犬，但是成功率仍较高，是建模的理想动物。
목적：비교개입법건립비격견여소형저량조동물급성심기경사모형적성공솔급풍험，위선택경합괄적동물건모제공이론기출。방법비격견화오지산소형저각6두，전신마취하행우측고동맥절개천자술，좌관상동맥조영후선택관상동맥구낭송지전강지중단행결혈예괄응，병지속봉도180 min후철출구낭，술전、술후균련속관찰심전도변화，술후7 d처사동물취출심장행병이학검사。결과비격견조균존활，소형저조사망1례（1／6）；비격견조발생심기경사1례（1／6），소형저조5례（5／6）；비격견조미발생악성심률실상，소형저조6례균발생악성심률실상（6／6）；량조조모소수시간비교[（4．61±0．47）h vs．（4．82±0．55）h]，차이무통계학의의（P＞0．05）。결론소형저재개입법건립심기경사모형중수연풍험고우비격견，단시성공솔잉교고，시건모적이상동물。
Objective To explore the success rate and the risk of establishment of the acute myocardial infarction model between the beagle dogs and the mini-pigs by interventional technique ,further to provid theoretical basis for choose a more suitable animal model .Methods 6 dogs and 6 mini-pigs were anaesthetized ,then underwent the coronary arteriography via femoral artery .After is-chemic preconditioning the coronary balloon was inflated to occlude the middle left anterior descending coronary for 180 minutes . The electrocardiogram was examined throughout the operation and the pathological sections were examined until the animals were executed one week later .Results All beagle dogs survived ,while 1 case of mini-pigs dead(1/6) .There was 1 cases(1/6) of beagle dogs had acute myocardial infarction ,while 5(5/6)cases in mini-pigs .All mini-pigs had malignant arrhythmia(6/6) but never seen in beagle dogs .The time needed for building a model was similar between the two groups ,the difference had no statistical signifi-cance(P>0 .05) .Conclusion The risk of establish myocardial infarction model in mini-pigs is higher than beagle dogs ,but the suc-cess rate is still high ,it might be the better choice .