CAJ | 학술논문

目的：评估新型生物人工肝大动物实验的安全性。方法将6只西藏猪采用混合与单纯两种生物治疗模式，观察记录实验过程中每小时生命体征、静脉压、跨膜压、入浆壶压的变化，并在实验开始时及实验4、8h抽取静脉端血做内毒素及血培养检测。结果生物人工肝机器的静脉压、跨膜压、入浆壶压值在实验5 h较实验开始时明显增高（P＜0．05），而其余各时间点与实验开始时比较差异无统计学意义（P＞0．05）。各时间点平均动脉压、呼吸频率比较差异无统计学意义（P＞0．05），实验2 h心率与实验开始时比较差异有统计学意义（P＜0．01），实验开始时及实验4、8 h抽血所测得的内毒素结果均小于0．5 EU／mL ，血培养结果显示培养15 d未见细菌生长。结论新一代生物人工肝大动物实验安全，可以进一步评价猪的肝衰竭模型的有效性。
목적：평고신형생물인공간대동물실험적안전성。방법장6지서장저채용혼합여단순량충생물치료모식，관찰기록실험과정중매소시생명체정、정맥압、과막압、입장호압적변화，병재실험개시시급실험4、8h추취정맥단혈주내독소급혈배양검측。결과생물인공간궤기적정맥압、과막압、입장호압치재실험5 h교실험개시시명현증고（P＜0．05），이기여각시간점여실험개시시비교차이무통계학의의（P＞0．05）。각시간점평균동맥압、호흡빈솔비교차이무통계학의의（P＞0．05），실험2 h심솔여실험개시시비교차이유통계학의의（P＜0．01），실험개시시급실험4、8 h추혈소측득적내독소결과균소우0．5 EU／mL ，혈배양결과현시배양15 d미견세균생장。결론신일대생물인공간대동물실험안전，가이진일보평개저적간쇠갈모형적유효성。
Objective To evaluate the security of big animals with a new bioartificial liver system .Methods Six Tibet pigs re-spectively received treatment of hybrid artificial liver and simple bioartificial liver ,observed and recorded the vital signs ,venous pressure ,transmembrane pressure and slurry pot pressure each hour ,and collected blood to make endotoxin and bacterial culture test in the zero hour ,fourth and eighth hour .Results Compared with the zero hour ,venous pressure ,transmembrane pressure ,slur-ry pot pressure were much higher in the fifth hour (P< 0 .05) ,and there were no significant difference in the rest of other time points(P>0 .05) .The mean arterial pressure and respiratory rate in all time point showed no significant changes (P>0 .05) .Com-pared with the zero hour ,the heart rate was much lower in the second hour (P<0 .01) .The values of blood endotoxin were less than 0 .5 EU/mL in the zero hour ,fourth and eighth hour from beginning ,and the bacterial culture test showed no growth of bacteria . Conclusion The experiment of big animals with a new bioartificial liver system was safe ,the efficacy in the treatment of hepatic failure could be assessed further .