CAJ | 학술논문

目的：检测转录因子Blimp1在潜伏期、活动期结核病患者及健康者中的表达差异，评估其作为新型结核病诊断因子的可能性。方法选取60例活动期结核病患者（活动期组）、50例潜伏期结核病患者（潜伏期组）和50例健康体检者（对照组）作为研究对象，利用荧光定量PCR方法分别测定3组研究对象外周血单个核细胞中Blimp1 mRNA的表达情况。结果活动期组Blimp1 mRNA的表达量是对照组的15．35倍，是潜伏期组的2．21倍，组间差异有统计学意义（P＜0．05）。结论 Blimp1参与抗结核免疫，有助于区分活动性肺结核和健康人群，为结核病的发病机制提供新的实验依据，从而进一步为结核病的早期诊断和治疗提供新的方向。
목적：검측전록인자Blimp1재잠복기、활동기결핵병환자급건강자중적표체차이，평고기작위신형결핵병진단인자적가능성。방법선취60례활동기결핵병환자（활동기조）、50례잠복기결핵병환자（잠복기조）화50례건강체검자（대조조）작위연구대상，이용형광정량PCR방법분별측정3조연구대상외주혈단개핵세포중Blimp1 mRNA적표체정황。결과활동기조Blimp1 mRNA적표체량시대조조적15．35배，시잠복기조적2．21배，조간차이유통계학의의（P＜0．05）。결론 Blimp1삼여항결핵면역，유조우구분활동성폐결핵화건강인군，위결핵병적발병궤제제공신적실험의거，종이진일보위결핵병적조기진단화치료제공신적방향。
Objective To analyze Blimp1 expression difference in the latent and active of tuberculosis patients and the healthy , and assess possibility of as the new tuberculosis diagnostics molecules .Methods 60 patients with active TB (active tuberculosis group) ,50 participants with latent(latent tuberculosis group) ,and 50 healthy people(control group) were enrolled separately .Using fluorescence quantitative polymerase chain reaction were used to determin Blimp 1 mRNA expression in the peripheral blood mono-nuclear cells .Results Blimp1 mRNA expression level of the active tuberculosis group was 15 .35 times than the control group ,and 2 .21 times than the latent tuberculosis group ,the difference was statistically significant (P<0 .05) .Conclusion Blimp1 gene proba-bly plays a role in the immune response to tuberculosis .it provides new ideas for the laboratory diagnosis of tuberculosis and new clues for further exploring the pathogenesis mechanism of tuberculosis .