漳州师范学院学报:自然科学版
漳州師範學院學報:自然科學版
장주사범학원학보:자연과학판
Journal of ZhangZhou Teachers College(Natural Science)
2012年
2期
90-95
,共6页
冷波%陈芸芸%郑艺梅%卢燕%黄妇仔
冷波%陳蕓蕓%鄭藝梅%盧燕%黃婦仔
랭파%진예예%정예매%로연%황부자
胰蛋白酶%水解条件%丈蛤
胰蛋白酶%水解條件%丈蛤
이단백매%수해조건%장합
Parenzyme%optimal hydrolysis conditions%Meretrix Meretrix Linnaeus
研究了胰蛋白酶水解文蛤肉制备多肽的最适条件,通过单因素实验和正交实验研究几种影响因素(pH、温度,时间和酶量)对胰蛋白酶水解文蛤肉的影响,分析探讨了影响胰蛋白酶水解文蛤肉的关键因素并确定胰蛋白酶水解文蛤肉的最佳水解条件.结果表明,采用pH8.5、温度50℃、加酶量:(87500:1)U/g、反应时闻5h的水解条件,利用胰蛋白酶水解文蛤肉悬液,可以得到分子量分布在500-3500Da范围内的文蛤多肽.
研究瞭胰蛋白酶水解文蛤肉製備多肽的最適條件,通過單因素實驗和正交實驗研究幾種影響因素(pH、溫度,時間和酶量)對胰蛋白酶水解文蛤肉的影響,分析探討瞭影響胰蛋白酶水解文蛤肉的關鍵因素併確定胰蛋白酶水解文蛤肉的最佳水解條件.結果錶明,採用pH8.5、溫度50℃、加酶量:(87500:1)U/g、反應時聞5h的水解條件,利用胰蛋白酶水解文蛤肉懸液,可以得到分子量分佈在500-3500Da範圍內的文蛤多肽.
연구료이단백매수해문합육제비다태적최괄조건,통과단인소실험화정교실험연구궤충영향인소(pH、온도,시간화매량)대이단백매수해문합육적영향,분석탐토료영향이단백매수해문합육적관건인소병학정이단백매수해문합육적최가수해조건.결과표명,채용pH8.5、온도50℃、가매량:(87500:1)U/g、반응시문5h적수해조건,이용이단백매수해문합육현액,가이득도분자량분포재500-3500Da범위내적문합다태.
In this present, Optimum hydrolysis conditions of Parenzyme to the Meretrix Meretrix Linnaeus are studied. Several factors such as pH, temperature, time and enzymes dosage of the hydrolysis are determined by single factor experiments and orthogonal experiment. The results show that optimal hydrolysis conditions of Parenzyme to the Meretrix Meretrix Linnaeus are respectively pH 8.5, temperature of 50℃, enzymes dosage of (87500:1) U / g, reaction time of 5h, and peptides of low molecular weight of 500-3500 Da can be prepared effectively.