CAJ | 학술논문

从河南南阳宝天曼森林土壤样品中分离纯化细菌，以对线虫Caenorhabditis elegans的杀灭作用为筛选模型，从128株细菌中共筛选到3株杀线虫活性较高的细菌，编号分别为B、C、D。当作用于线虫24h后，它们对线虫的致死率分别为20％、25％、97％。然而发现当B和C共同作用于线虫时，致死率大为提高（约为90％），说明二者在杀线虫方面有协同作用。通过培养观察菌落形态，革兰氏染色；提取各菌种基因组，扩增16SrDNA测序，系统发育分析，确定这三株菌的鉴定结果为：菌株B与Bacillus subtilis strain xfchu2的同源性较高，为96．46％，菌株C与Stenotrophomonas maltophilia strain QT24的同源性较高，为92．75％。菌株D与荧光假单胞菌属的同源性较高。
종하남남양보천만삼림토양양품중분리순화세균，이대선충Caenorhabditis elegans적살멸작용위사선모형，종128주세균중공사선도3주살선충활성교고적세균，편호분별위B、C、D。당작용우선충24h후，타문대선충적치사솔분별위20％、25％、97％。연이발현당B화C공동작용우선충시，치사솔대위제고（약위90％），설명이자재살선충방면유협동작용。통과배양관찰균락형태，혁란씨염색；제취각균충기인조，확증16SrDNA측서，계통발육분석，학정저삼주균적감정결과위：균주B여Bacillus subtilis strain xfchu2적동원성교고，위96．46％，균주C여Stenotrophomonas maltophilia strain QT24적동원성교고，위92．75％。균주D여형광가단포균속적동원성교고。
Nematocidal bacteria were screened and isolated from Baotianman' s forest soil sample of Nanyang, Henan. Based on the model of killing effect on Caenorhabditis elegans, three bacteria were selected from 128 strains of bacteria, which had high activity of killing nematodes and were separately numbered B, C, D. When they treated with nematodes for 24 h, the mortalities of nematodes were 20% ,25% and 97%. However, the mortality of nematodes could be greatly improved when B and C interacted with nematodes together, which indicated that the two bacteria killed nematodes synergistically. The morphological characteristics of the bacteria were analyzed through Gram stain. The 16S rDNA sequences of the bacteria were obtained by PCR. The results of the molecular identifications of the three bacteria were as follows: strain B had high homologous with Bacillus subtilis strain xfchu2 (96.46%) ; strain C had high homologous with Stenotrophomonas maltophilia strain QT24 (92.75%) ; strain D had high homologous with Pseudomonas fluorscen.