西南科技大学学报
西南科技大學學報
서남과기대학학보
JOURNAL OF SOUTHWEST CHINA INSTITUTE OF TECHNOLOGY
2012年
4期
94-100
,共7页
直接优化%常规分析%膜盘菌属%系统进化树
直接優化%常規分析%膜盤菌屬%繫統進化樹
직접우화%상규분석%막반균속%계통진화수
Direct optimization%Classical analysis%Hymenoscyphus%Phylogenetic tree
以rDNA ITSl—5．8S—ITS2序列作为分子标记，采用直接优化和常规分析两种方法，探讨膜盘菌属内种间及其与相关属间的亲缘关系，并比较了两种建树方法在解决该属内种间关系的效果。以Lachnum spartinae为外群，选取31条序列构建了最小进化树、最大简约树和直接优化树。结果表明，Hymenoscyphus ericae复合种与其他膜盘菌属的成员关系较远，除了Hericae外，其他膜盘菌形成一个单系群。模式种Hymenoscyphus fructigenus与Hcaudatus，Hfucatus，Hscutulus等构成一个高支持率的分支，与另一分支（Hepiphyllus，Hlasiopodium）形成姐妹群。相对于常规的两步分析法，直接优化所产生的系统树与当前形态学特征基本相符合，因而更合理地反映膜盘菌属的分类地位和种间关系，表明该分析方法在真菌系统发育分析中具有较大的应用价值。
以rDNA ITSl—5．8S—ITS2序列作為分子標記，採用直接優化和常規分析兩種方法，探討膜盤菌屬內種間及其與相關屬間的親緣關繫，併比較瞭兩種建樹方法在解決該屬內種間關繫的效果。以Lachnum spartinae為外群，選取31條序列構建瞭最小進化樹、最大簡約樹和直接優化樹。結果錶明，Hymenoscyphus ericae複閤種與其他膜盤菌屬的成員關繫較遠，除瞭Hericae外，其他膜盤菌形成一箇單繫群。模式種Hymenoscyphus fructigenus與Hcaudatus，Hfucatus，Hscutulus等構成一箇高支持率的分支，與另一分支（Hepiphyllus，Hlasiopodium）形成姐妹群。相對于常規的兩步分析法，直接優化所產生的繫統樹與噹前形態學特徵基本相符閤，因而更閤理地反映膜盤菌屬的分類地位和種間關繫，錶明該分析方法在真菌繫統髮育分析中具有較大的應用價值。
이rDNA ITSl—5．8S—ITS2서렬작위분자표기，채용직접우화화상규분석량충방법，탐토막반균속내충간급기여상관속간적친연관계，병비교료량충건수방법재해결해속내충간관계적효과。이Lachnum spartinae위외군，선취31조서렬구건료최소진화수、최대간약수화직접우화수。결과표명，Hymenoscyphus ericae복합충여기타막반균속적성원관계교원，제료Hericae외，기타막반균형성일개단계군。모식충Hymenoscyphus fructigenus여Hcaudatus，Hfucatus，Hscutulus등구성일개고지지솔적분지，여령일분지（Hepiphyllus，Hlasiopodium）형성저매군。상대우상규적량보분석법，직접우화소산생적계통수여당전형태학특정기본상부합，인이경합리지반영막반균속적분류지위화충간관계，표명해분석방법재진균계통발육분석중구유교대적응용개치。
The phylogeny of the genus Hymenoscyphus and related genera by using rDNA ITS1 -5.8S - ITS2 region. Two kinds of methods, Direct Optimization and Classical Analysis, were applied in this study, and the performances of classical analysis and direct optimization were evaluated. 31 sequences were selected, and three phylogenetic trees (maximum parsimony tree, minimum evolution tree and di- rect optimization tree) were reconstructed with Lachnum spartinae as outgroup. The results indicate that the Hymenoscyphus was monophyly with exclusion of H. ericae. Hymenoscyphus fructigenus, the lecto- type of the genus, together with H. caudatus, H. fucatus, H. scutulus, represented a monophyletic group with high bootstrap values, which was a sister group to H. epiphyllus -H. lasiopodium. In con- trast to the conventional two-step analysis method, the phylogeny based on Direct Optimization is better correlation with currently phenotypic features, which reasonably showed the taxonomic position of the ge- nus Hymenoscyphus, as well as phylogenetic relationships among Hymenoscyphus speices. Therefore, our results demonstrated a certain superiority of the application of Direct Optimization method in phylogenetic analyses of fungi.
