CAJ | 학술논문

对提取β-胡萝卜素后的杜氏盐藻渣中多糖的提取、初步纯化和多糖脱色方法进行了实验研究.实验采用水浸提的方法,考察了提取温度、提取时间、液固比和pH值对盐藻多糖提取率的影响,盐藻多糖的最适提取条件为：提取温度：70℃,提取时间：4 h,液固比：30：1,pH值10,在此条件下,盐藻多糖的提取率为8.63%,多糖含量为65.73%.采用分级醇沉和分步醇沉的方法沉淀多糖,得到醇沉最佳条件为无水乙醇一步醇沉.采用中性蛋白酶水解脱除盐藻多糖提取液中蛋白质,脱除率为61.55%,脱蛋白后多糖含量为70.96%,采用过氧化氢对脱蛋白后多糖提取液脱色,脱色率为78.66%.
대제취β-호라복소후적두씨염조사중다당적제취、초보순화화다당탈색방법진행료실험연구.실험채용수침제적방법,고찰료제취온도、제취시간、액고비화pH치대염조다당제취솔적영향,염조다당적최괄제취조건위：제취온도：70℃,제취시간：4 h,액고비：30：1,pH치10,재차조건하,염조다당적제취솔위8.63%,다당함량위65.73%.채용분급순침화분보순침적방법침정다당,득도순침최가조건위무수을순일보순침.채용중성단백매수해탈제염조다당제취액중단백질,탈제솔위61.55%,탈단백후다당함량위70.96%,채용과양화경대탈단백후다당제취액탈색,탈색솔위78.66%.
The extraction technology,primary purity method and decolorization method of polysaccharide from Dunaliella salina residue after β-carotene are extracted.A water immersion extraction method was adopted in this experiment,the effects of extraction temperature,extraction time,material to liquid ratio,pH value on extraction efficiency of D.salina polysaccharide were investigated.The optimal extraction condition was extraction temperature 70℃,extraction time 4 h,material to liquid ratio 30 ml/g,pH 10,under this condition,the extraction ratio was 8.63% and the polysaccharide concentration was 65.73%.The optimal precipitation condition of polysaccharide was adopted the way of once alcohol-precipitation by 100% ethanol(v/v).The deproteinizing ratio of polysaccharide extracts was 61.55% after hydrolyzed by neutral protease,its polysaccharide content is 70.96%,and the decolorization ratio was 78.66% by hydrogen peroxide.