CAJ | 학술논문

取3～5mg花生子叶组织采用简化的DNA提取流程和快速银染方法，鉴定了利用耐低温花生品系A4搭配的两个杂交组合的F1真杂种种子。本研究为下一步阐明花生耐低温特性的遗传规律奠定了基础，对花生高产稳产育种具有重要意义。
취3～5mg화생자협조직채용간화적DNA제취류정화쾌속은염방법，감정료이용내저온화생품계A4탑배적량개잡교조합적F1진잡충충자。본연구위하일보천명화생내저온특성적유전규률전정료기출，대화생고산은산육충구유중요의의。
By using a simplified DNA extraction protocol with 3-5 mg groundnut cotyledon- ary tissue and a quick silver-staining procedure, true F1 hybrid seeds were identified by SSR analysis in two cross combinations involving a chilling tolerant line, A4. As the first step to- wards elucidation of chilling tolerance genetics in groundnut, this study is also of relevance to groundnut breeding for stable and high yields.