CAJ | 학술논문

对河北省不同地区发病猪场分离的已经过系统鉴定的12株猪源大肠杆菌，采用K。B纸片法进行了15种抗生素的耐药性检测，并提取质粒，对耐药谱和质粒谱型进行了比较分析。结果显示，分离菌株对氨苄西林的耐药率达到了100％，对阿莫西林的耐药率为91．7％，对四环素和复方新诺明的耐药率介于50％。70％。分离菌株存在多重耐药性，以耐4种、6种和7种药物为主，占总数的75％。对分离菌株耐药谱与质粒谱的分析表明，大肠杆菌的质粒携带与细菌的耐药性之间并没有明显的对应关系。
대하북성불동지구발병저장분리적이경과계통감정적12주저원대장간균，채용K。B지편법진행료15충항생소적내약성검측，병제취질립，대내약보화질립보형진행료비교분석。결과현시，분리균주대안변서림적내약솔체도료100％，대아막서림적내약솔위91．7％，대사배소화복방신낙명적내약솔개우50％。70％。분리균주존재다중내약성，이내4충、6충화7충약물위주，점총수적75％。대분리균주내약보여질립보적분석표명，대장간균적질립휴대여세균적내약성지간병몰유명현적대응관계。
In this experiment,the antimicrobial susceptibilities to 15 antibiotics of 12 pathogenic Escherichia co- li from piglets of different cities and counties in Hebei province were determined by Kirby-Bauer method. The plasmid was extracted and 12 drug-resistant strains were analyzed for molecular typing and plasmid homology cluster. The results showed that: 100 resistance rate against ampicillin and 91.7 against Amoxicillin. The re- sistance rate against tetracyclineand trimethoprim and sulphame-thoxazole were 50%- 70%. Multi-drug resist- ance test showed that 75% E. coli strains were 4,6 and 7 kinds of antibiotics resistance. The analysis of com- parative resistance spectra and plasmid profile showed that there was no direct relationship between the drug resistance and the number and size of plasmids.