CAJ | 학술논문

采用生物化学方法中的有机溶剂分级沉淀法，对碱性磷酸酶进行了分离纯化，用金氏法测定其活性，并用Folin-酚法测定酶含量，然后筛选碱性磷酸酶的最适pH值和最适温度。在此条件下研究不带电荷极性氨基酸对兔肝中碱性磷酸酶活性的影响。结果表明，兔肝中碱性磷酸酶的最适pH值为9．84，最适温度为39℃。在不带电荷极性氨基酸中甘氨酸、苏氨酸、半胱氨酸和天冬酰胺对碱性磷酸酶的活性有不同的抑制作用，而丝氨酸对碱性磷酸酶的活性有激活作用，这说明不带电荷极性氨基酸对酶活性抑制或激活作用与其侧链基团有关。
채용생물화학방법중적유궤용제분급침정법，대감성린산매진행료분리순화，용금씨법측정기활성，병용Folin-분법측정매함량，연후사선감성린산매적최괄pH치화최괄온도。재차조건하연구불대전하겁성안기산대토간중감성린산매활성적영향。결과표명，토간중감성린산매적최괄pH치위9．84，최괄온도위39℃。재불대전하겁성안기산중감안산、소안산、반광안산화천동선알대감성린산매적활성유불동적억제작용，이사안산대감성린산매적활성유격활작용，저설명불대전하겁성안기산대매활성억제혹격활작용여기측련기단유관。
Alkaline phosphatase (AP) was separated and depurated with fractional precipitation of organic sol- vent from liver of rabbit. The AP activity was measured with the King' s Method, and the content of enzyme was detected with the Folin-phenol' s Method, in order to determine the optimum pH and temperature, for AP activity determination and investigate the effects of polar uncharged amino acids on liver AP activity of rabbit. The results showed that the optimum pH and temperature for AP activity determination was 9.84 and 39℃ , respectively. Threonine, cysteine, aspartic acid, and glutanic acid had inhibitory effects on liver AP activety, and serine had activated effect on liver AP activity. These results impiied that the actions of polar uncharged a- mino acids on liver AP activity were related with their side chains.