CAJ | 학술논문

以红树植物秋茄叶片为实验材料,美人蕉叶片为对照材料,采用6种不同的DNA提取方法,比较不同方法的DNA提取效果。结果表明：针对红树植物优化的CTAB法（方法 6）通过PVP的除酚去多糖作用,利用CTAB提取液和氯仿-异戊醇强效除蛋白和脂类物质,结合高盐环境下异丙醇高效沉淀DNA去除多糖,最终达到彻底去除杂质,得到高纯度的基因组DNA,是6种方法中最适合用于红树植物DNA的提取方法。
이홍수식물추가협편위실험재료,미인초협편위대조재료,채용6충불동적DNA제취방법,비교불동방법적DNA제취효과。결과표명：침대홍수식물우화적CTAB법（방법 6）통과PVP적제분거다당작용,이용CTAB제취액화록방-이무순강효제단백화지류물질,결합고염배경하이병순고효침정DNA거제다당,최종체도철저거제잡질,득도고순도적기인조DNA,시6충방법중최괄합용우홍수식물DNA적제취방법。
In the present study, we used 6 different methods to extract DNA from the leave of the mangrove Kandelia obovata. By comparing different methods of DNA extraction, we found that: the modified CTAB method (Mothod.6 in this paper), by using PVP and 5 mol/L NaC1 to get rid of tannins and polysaecharides, together with the function of CTAB buffer, Chloroform: Isoamyl alcohoL(24:l) and Isopropanol, successfully purified the DNA, thus to made it the most suitable method for extracting DNA from the mangrove material.