CAJ | 학술논문

采用不连续聚丙烯酰胺垂直板凝胶电泳方法,对不同浓度Hg2＋对果蝇生育力、寿命和酯酶活性及其同工酶表达的影响.结果表明：不同浓度的Hg2＋对果蝇的生育力和寿命均有影响,产生出不同酶谱,其变化为酶带数、着色度、酶相对活性的不同.说明随着Hg2＋浓度的升高,果蝇的生育力降低、寿命缩短;果蝇幼虫体内的EST酶相对活性发生变化,同工酶表达受到显著的影响.
채용불련속취병희선알수직판응효전영방법,대불동농도Hg2＋대과승생육력、수명화지매활성급기동공매표체적영향.결과표명：불동농도적Hg2＋대과승적생육력화수명균유영향,산생출불동매보,기변화위매대수、착색도、매상대활성적불동.설명수착Hg2＋농도적승고,과승적생육력강저、수명축단;과승유충체내적EST매상대활성발생변화,동공매표체수도현저적영향.
This paper using discontinuous polyacrylamide gel electrophoresis method,with different concentrations of Hg2+ on Drosophila melanogaster reproductive capacity,longevity and esterase activity and its isozyme expression.The results showed that,different concentrations of Hg2 + on Drosophila melanogaster reproductive capacity and longevity are affected,produce different zymogram,change as the enzyme bands,coloring degree and different relative activity.With the Hg2 + concentration increasing the Drosophila melanogaster of reproductive capacity is reduce,life expectancy decurtation;Drosophila melanogaster larvae EST relative activity changes,isozyme expression have been significantly affected.