CAJ | 학술논문

通过单因素试验和正交试验分析料液比、浸提温度、浸提时间和浸提次数对人工北虫草僵虫（菌核）粗多糖得率的影响,结果表明：随浸提温度、浸提时间和浸提次数的增加,粗多糖得率逐渐上升;随料液比提高,粗多糖得率呈先上升后下降的趋势;影响粗多糖得率的主效因素是浸提温度,其次为浸提时间和浸提次数;确定最佳浸提条件为：浸提温度100℃,料液比1∶20,浸提时间3h,浸提2次,在此条件下北虫草僵虫粗多糖得率为8.497%.
통과단인소시험화정교시험분석료액비、침제온도、침제시간화침제차수대인공북충초강충（균핵）조다당득솔적영향,결과표명：수침제온도、침제시간화침제차수적증가,조다당득솔축점상승;수료액비제고,조다당득솔정선상승후하강적추세;영향조다당득솔적주효인소시침제온도,기차위침제시간화침제차수;학정최가침제조건위：침제온도100℃,료액비1∶20,침제시간3h,침제2차,재차조건하북충초강충조다당득솔위8.497%.
In this paper, effects of solid-liquid ratio,temperature, extracting period, and extracting times on crude polysaccharide yields in cultured C. rnilitaris sclerotium are investigated by using one-factor experimental design and orthogonal test design. One-factor experimental results implied that crude polysaccharides rise as temperature, extracting period, and number of extraction times increase, while crude polysaccharides firstly rise and then drop as solid-liquid ratio changing. The results of orthogonal test showed that the main factor affecting yields of polysaccharide is temperature, and then extracting period and number of extracting times. An optimal isolation condition of 100℃, a solid-liquid ratio of 1 : 20(g/mL), 3 hour's extracting, and 2 extractings is achieved,which yields a polysaccharide content of 8. 497% in C. militaris sclerotium.