CAJ | 학술논문

以龙竹和凤尾竹基因组DNA为模板,通过试验不同的退火温度、循环数、Mg2＋浓度及DMSO浓度,获得了16条龙竹和凤尾竹PpMADS1相似序列片段,并得出稳定扩增PpMADS1相似序列的条件.对获得的龙竹和凤尾竹PpMADS1相似序列进行序列分析,发现龙竹和凤尾竹PpMADS1相似序列可根据序列长度差异分成组I和组II,并对其推出的氨基酸序列进行分析,找到了保守的paleoAP1基序和KIII区保守的氨基酸残基,并据此认为龙竹和凤尾竹PpMADS1相似序列是AP1/SQUA亚家族成员.
이룡죽화봉미죽기인조DNA위모판,통과시험불동적퇴화온도、순배수、Mg2＋농도급DMSO농도,획득료16조룡죽화봉미죽PpMADS1상사서렬편단,병득출은정확증PpMADS1상사서렬적조건.대획득적룡죽화봉미죽PpMADS1상사서렬진행서렬분석,발현룡죽화봉미죽PpMADS1상사서렬가근거서렬장도차이분성조I화조II,병대기추출적안기산서렬진행분석,조도료보수적paleoAP1기서화KIII구보수적안기산잔기,병거차인위룡죽화봉미죽PpMADS1상사서렬시AP1/SQUA아가족성원.
In this paper, annealing temperature, number of cycles, concentration of Mg2+, and concentration of DMSO are investigated to find a steady procedure for PpMADSI-like sequence amplification through Polymerase Chain Reaction (PCR) and finally sixteen PpMADSI-like sequences are obtained using genomic DNA as template DNA in Dendrocalamus giganteus and Bambusa multiplex cv. fernlea{. All sequences obtained are sorted into two groups~ group I and group II, based on sequence length polymorphism and the results of sequence analysis of putative amino acid sequences. Conserved paleoAP1 motif and conserved amino acid residues of KIII region of K box are also observed, according to which all PpMADSI-like sequences are believed to be members of AP1/SQUA subfamily of MIKC-type MADS-box gene family.