CAJ | 학술논문

建立高效液相色谱检测冷饮中靛蓝含量的方法，样品中的靛蓝经三氯甲烷超声波提取，提取液浓缩后用甲醇-异丙醇（1＋1）溶解和定容，注入高效液色谱仪检测分析。在所采用的色谱条件下，靛蓝在1～30μg／ml间线性方程的相关系数可达0．9999，重现性的标准偏差为2．35％（n=6），精密度为0．23％，检出限为0．3mg／kg，加标回收率在84％-109％之间。该方法操作简单快捷，结果准确，令人满意。
건립고효액상색보검측랭음중전람함량적방법，양품중적전람경삼록갑완초성파제취，제취액농축후용갑순-이병순（1＋1）용해화정용，주입고효액색보의검측분석。재소채용적색보조건하，전람재1～30μg／ml간선성방정적상관계수가체0．9999，중현성적표준편차위2．35％（n=6），정밀도위0．23％，검출한위0．3mg／kg，가표회수솔재84％-109％지간。해방법조작간단쾌첩，결과준학，령인만의。
A method was developed for determination of indigo contents in cold drinks. The indigo in sample was extracted by tricbloromethane ultrasonic wave, concentrated, dissolved to become a constant volume by methanol-i- sopropyl alcohol, and analyed by HPLC. The results showed that the liner correlation of indigo was up to 0. 9999 in the ranges 1.0 - 30.0 μg/mL, the standard deviation of reproducibility was 2.35 % (n = 6) , the RSD was 0.23 % , the detection limit was 0.3 mg/kg, and the recovery rate ranged from 84 % to 109 %. The method is rapid, accurate and satisfactory.