北华大学学报：自然科学版
北華大學學報：自然科學版
북화대학학보：자연과학판
Journal of Beihua University(Natural Science)
2012年
6期
648-650
,共3页
bFGF%慢性应激%学习记忆%Ach
bFGF%慢性應激%學習記憶%Ach
bFGF%만성응격%학습기억%Ach
basic fibroblast growth factor%chronic stress mice%learning-memory%Ach
目的探讨碱性成纤维细胞生长因子（Basic fibroblast growth factor,bFGF）对慢性应激模型小鼠学习记忆的影响及海马内Ach含量的变化.方法利用不确定应激方法建立慢性应激动物模型,共4周;应激第15天开始每日应激前腹腔注射bFGF,持续15 d;应用跳台法和避暗法观察bFGF对慢性应激小鼠学习记忆的作用;碱羟胺比色法检测乙酰胆碱（Ach）含量.结果 bFGF组小鼠学习记忆能力提高,海马Ach含量增高.结论 bFGF通过增加海马内胆碱能功能,改善慢性应激小鼠学习记忆能力.
目的探討堿性成纖維細胞生長因子（Basic fibroblast growth factor,bFGF）對慢性應激模型小鼠學習記憶的影響及海馬內Ach含量的變化.方法利用不確定應激方法建立慢性應激動物模型,共4週;應激第15天開始每日應激前腹腔註射bFGF,持續15 d;應用跳檯法和避暗法觀察bFGF對慢性應激小鼠學習記憶的作用;堿羥胺比色法檢測乙酰膽堿（Ach）含量.結果 bFGF組小鼠學習記憶能力提高,海馬Ach含量增高.結論 bFGF通過增加海馬內膽堿能功能,改善慢性應激小鼠學習記憶能力.
목적탐토감성성섬유세포생장인자（Basic fibroblast growth factor,bFGF）대만성응격모형소서학습기억적영향급해마내Ach함량적변화.방법이용불학정응격방법건립만성응격동물모형,공4주;응격제15천개시매일응격전복강주사bFGF,지속15 d;응용도태법화피암법관찰bFGF대만성응격소서학습기억적작용;감간알비색법검측을선담감（Ach）함량.결과 bFGF조소서학습기억능력제고,해마Ach함량증고.결론 bFGF통과증가해마내담감능공능,개선만성응격소서학습기억능력.
Objective To investigate effects of basic fibroblast growth factor(bFGF)on the ability of learning and memory,and the content of Ach in hippocampus in chronic stress mice.Method An uncertain stress method was used for four weeks to establish the chronic stress model in mice.bFGF was injected intraperitoneally for 15 days on 15th day of the stress.The step-down test and darkness avoidance test were used to observe the effect of bFGF on learning-memory ability in the chronic stress mice.The change of Ach levels was tested with the hydroxylamine colorimetric assay.Results The results showed that the learning-memory abilities of mice in bFGF group were improved and contents of Ach in hippocampus increased.Conclusion bFGF can improve the learning-memory ability of chronic stress mice through improving the cholinergic function in hippocampus.
