CAJ | 학술논문

以邻苯二酚为底物，研究了双核铜配合物模拟酪氨酸酶的活性。通过制备新鲜酪氨酸酶、β-环糊精与双核配合物的包合物、壳聚糖与双核配合物包合物，分别观察其与邻苯二酚的反应时间与吸收波长曲线。发现双核铜配合物与β-环糊精包合物溶液的吸收波长曲线与酪氨酸酶的吸收波长曲线相似，最大吸收波长也与酪氨酸酶相近，表明双核铜配合物与β-环糊精包合物具有与酪氨酸酶相似的活性，但活性较弱（酪氨酸酶的酶活是1400，双核铜配合物的酶活是200）；而双核铜配合物与壳聚糖的包合物没有酪氨酸酶的活性。
이린분이분위저물，연구료쌍핵동배합물모의락안산매적활성。통과제비신선락안산매、β-배호정여쌍핵배합물적포합물、각취당여쌍핵배합물포합물，분별관찰기여린분이분적반응시간여흡수파장곡선。발현쌍핵동배합물여β-배호정포합물용액적흡수파장곡선여락안산매적흡수파장곡선상사，최대흡수파장야여락안산매상근，표명쌍핵동배합물여β-배호정포합물구유여락안산매상사적활성，단활성교약（락안산매적매활시1400，쌍핵동배합물적매활시200）；이쌍핵동배합물여각취당적포합물몰유락안산매적활성。
The simulation of binuclear copper ( II ) complexes for the activity of tyrosinase was studied, through the preparation of the fresh tyrosinase, the two different inclusion complexes of binuclear copper ( II ) with β - cyclodextrin and chitosan, and then respective determination of their UV absorbance - time curves, with o - di- hydroxybenzene as the substrate. The result indicated that the activity of the binuclear copper ( II ) -β -cy- clodextrin) inclusion complex was similar to the tyrosinase, though its enzyme activity (200) was lower than the tyrosinase (1400) obviously. While the binuclear copper ( II ) - chitosan inclusion complex had no activity seemingly.