CAJ | 학술논문

目的：建立反相高效液相色谱法同时测定化妆品中的4种雌激素（雌酮、雌三醇、炔雌醇、已烯雌酚）的方法。方法：色谱柱Inertsil ODS—C18（4．6mm×250mm，5μm），流动相为甲醇-水（体积比为60：40），流速1mL／min，检测波长279nm，柱温：25℃。结果：4种雌激素成分在0．05-500μg／mL浓度范围内与其对应分峰面积线性关系良好。样品的平均加标回收率为97．3％-110．6％，RSD为2．9％-4．0％，化妆品中四种雌激素的检出限为0．02—0．03mg／kg。该方法操作简便、灵敏度高，能够同时检测化妆品中4种雌性激素的含量。
목적：건립반상고효액상색보법동시측정화장품중적4충자격소（자동、자삼순、결자순、이희자분）적방법。방법：색보주Inertsil ODS—C18（4．6mm×250mm，5μm），류동상위갑순-수（체적비위60：40），류속1mL／min，검측파장279nm，주온：25℃。결과：4충자격소성분재0．05-500μg／mL농도범위내여기대응분봉면적선성관계량호。양품적평균가표회수솔위97．3％-110．6％，RSD위2．9％-4．0％，화장품중사충자격소적검출한위0．02—0．03mg／kg。해방법조작간편、령민도고，능구동시검측화장품중4충자성격소적함량。
Object: To establish the RP - HPLC method for the simultaneous determination of four female sex hormones ( estrone, estriol, ethiny, diethylstilbestrol) in cosmetics. Method: The Intersil ODS - C18 (4. 6 mm × 250 nun, 5 μm) column was used; the' mobile phase consisted of methanol -water (60: 40) ; the flow rate was 1.0mL/min and the detection wavelength was at 279 nm; the column temperature was at25℃. Result: Four hormones showed good linear response in the range of 0. 05 - 500 μg/mL, LOD were 0. 02 - 0. 03mg/kg. The average recoveries were97. 3 - 110. 6%, and RSD were2. 9 -4. 0%. Conclusions : The results indicate that the method is simple, sensitive and can be used for the quality control of four hormones in cosmetics.